Abstract
This chapter gives examples of basic procedures of quantification of plant structures with use of image analysis, which are commonly employed to describe differences among experimental treatments or phenotypes of plant material. Tasks are demonstrated with the use of ImageJ, a widely used public domain Java image processing program. Principles of sampling design based on systematic uniform random sampling for quantitative studies of anatomical parameters are given to obtain their unbiased estimations and simplified “rules of thumb” are presented. The basic procedures mentioned in the text are: (1) sampling, (2) calibration, (3) manual length measurement, (4) leaf surface area measurement, (5) estimation of particle density demonstrated on an example of stomatal density, and (6) analysis of epidermal cell shape.
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Acknowledgments
The authors wish to acknowledge the funding by the projects GACR P501/10/0340, 18-23702S, by the Charles University in Prague, project SVV 265203, and by the Ministry of Education, Youth and Sports of the Czech Republic, project NPUI LO1417 and institutional support RVO:67985823. We thank Miroslav Barták for his technical help with graphical image processing.
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Albrechtová, J., Kubínová, Z., Soukup, A., Janáček, J. (2019). Image Analysis: Basic Procedures for Description of Plant Structures. In: Cvrčková, F., Žárský, V. (eds) Plant Cell Morphogenesis. Methods in Molecular Biology, vol 1992. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9469-4_7
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DOI: https://doi.org/10.1007/978-1-4939-9469-4_7
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