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Antisense Oligodeoxynucleotide-Mediated Gene Knockdown in Pollen Tubes

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1992)

Abstract

Specific gene knockdown mediated by the antisense oligodeoxynucleotides (AODNs) strategy emerged as a rapid and effective tool for probing gene role in plant cells, particularly tip-growing pollen tubes. Here, we describe the protocol for the successful employment of AODN technique in growing tobacco pollen tubes, covering AODN design, application, and analysis of the results. We also discuss the advantages and drawbacks of this method.

Key words

Antisense oligodeoxynucleotide Pollen tube Gene knockdown Tip growth 

Notes

Acknowledgments

Research in the authors’ lab is supported by Czech Science Foundation (grants no. 17-27477S, 18-18290J and 19-21758S) and by Ministry of Education Youth and Sport of the Czech Republic (project no. NPUI LO1417).

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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Institute of Experimental BotanyCzech Academy of SciencesPragueCzech Republic
  2. 2.Department of Experimental Plant Biology, Faculty of ScienceCharles UniversityPragueCzech Republic

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