Antisense Oligodeoxynucleotide-Mediated Gene Knockdown in Pollen Tubes
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Specific gene knockdown mediated by the antisense oligodeoxynucleotides (AODNs) strategy emerged as a rapid and effective tool for probing gene role in plant cells, particularly tip-growing pollen tubes. Here, we describe the protocol for the successful employment of AODN technique in growing tobacco pollen tubes, covering AODN design, application, and analysis of the results. We also discuss the advantages and drawbacks of this method.
Key wordsAntisense oligodeoxynucleotide Pollen tube Gene knockdown Tip growth
Research in the authors’ lab is supported by Czech Science Foundation (grants no. 17-27477S, 18-18290J and 19-21758S) and by Ministry of Education Youth and Sport of the Czech Republic (project no. NPUI LO1417).