Quantifying Plant Growth and Cell Proliferation with MorphoGraphX
Confocal microscopy is widely used to live-image plant tissue. Cell outlines can be visualized using fluorescent probes that mark the cell wall or plasma membrane, enabling the confocal microscope to be used as a 3D scanner with submicron precision. After imaging, the data needs to be analyzed by specialized software to quantify the features of interest, such as cell size and shape, growth rates and anisotropy, and gene expression. Here we present a protocol for the 3D image processing software MorphoGraphX (www.MorphoGraphX.org) using time-lapse images of an Arabidopsis thaliana sepal and the shoot apex of tomato.
Key wordsConfocal microscopy Image analysis 3D visualization Time lapse Cell proliferation Growth analysis Lineage tracking
We gratefully acknowledge all those involved in the development of the MorphoGraphX, with special thanks to Anne-Lise Routier-Kierzkowska. We acknowledge Pierre Barbier de Reuille and Sarah Robinson who contributed to a previous version of this chapter . We would also like to thank the Tsiantis’s department of the Max Planck Institute for Plant Breeding Research for support.
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