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Quantifying Plant Growth and Cell Proliferation with MorphoGraphX

  • Soeren Strauss
  • Aleksandra Sapala
  • Daniel Kierzkowski
  • Richard S. SmithEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1992)

Abstract

Confocal microscopy is widely used to live-image plant tissue. Cell outlines can be visualized using fluorescent probes that mark the cell wall or plasma membrane, enabling the confocal microscope to be used as a 3D scanner with submicron precision. After imaging, the data needs to be analyzed by specialized software to quantify the features of interest, such as cell size and shape, growth rates and anisotropy, and gene expression. Here we present a protocol for the 3D image processing software MorphoGraphX (www.MorphoGraphX.org) using time-lapse images of an Arabidopsis thaliana sepal and the shoot apex of tomato.

Key words

Confocal microscopy Image analysis 3D visualization Time lapse Cell proliferation Growth analysis Lineage tracking 

Notes

Acknowledgments

We gratefully acknowledge all those involved in the development of the MorphoGraphX, with special thanks to Anne-Lise Routier-Kierzkowska. We acknowledge Pierre Barbier de Reuille and Sarah Robinson who contributed to a previous version of this chapter [7]. We would also like to thank the Tsiantis’s department of the Max Planck Institute for Plant Breeding Research for support.

References

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Soeren Strauss
    • 1
  • Aleksandra Sapala
    • 2
    • 1
  • Daniel Kierzkowski
    • 1
    • 3
  • Richard S. Smith
    • 1
    Email author
  1. 1.Max Planck Institute for Plant Breeding ResearchCologneGermany
  2. 2.Department of Biosystems Science and EngineeringETH ZurichSwitzerland
  3. 3.Institut de Recherche en Biologie VégétaleUniversity of MontréalMontrealCanada

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