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Essential Methods of Plant Sample Preparation for Light Microscopy

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Plant Cell Morphogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1992))

Abstract

There are various preparatory techniques for light microscopy permitting access to the inner structure of plant body and its development. Minute objects might be processed as whole-mount preparations, while voluminous ones should be separated into smaller pieces. Here we summarize some of the “classical” techniques to cut more voluminous objects into slices and access their inner structure either for simple anatomical analysis or for further processing (e.g., histochemistry, immunohistochemistry, in situ hybridization, enzyme histochemistry).

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Acknowledgments

The current update of this chapter, originally published as ref. 47, has been supported by the NPUI-LO1417 project.

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Authors and Affiliations

  1. Department of Experimental Plant Biology, Faculty of Science, Charles University, Prague, Czech Republic

    Aleš Soukup & Edita Tylová

Authors
  1. Aleš Soukup
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  2. Edita Tylová
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Corresponding author

Correspondence to Aleš Soukup .

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Editors and Affiliations

  1. Department of Experimental Plant Biology, Charles University, Prague, Czech Republic

    Fatima Cvrčková

  2. Department of Experimental Plant Biology, Charles University, Prague, Czech Republic

    Viktor Žárský

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Soukup, A., Tylová, E. (2019). Essential Methods of Plant Sample Preparation for Light Microscopy. In: Cvrčková, F., Žárský, V. (eds) Plant Cell Morphogenesis. Methods in Molecular Biology, vol 1992. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9469-4_1

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  • DOI: https://doi.org/10.1007/978-1-4939-9469-4_1

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9468-7

  • Online ISBN: 978-1-4939-9469-4

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