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Protein Acetylation in Pathogen Virulence and Host Defense: In Vitro Detection of Protein Acetylation by Radiolabeled Acetyl Coenzyme A

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Plant Innate Immunity

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1991))

Abstract

Protein acetylation has emerged as a common modification that modulates multiple aspects of protein function, including localization, stability, and protein-protein interactions. It is increasingly evident that protein acetylation significantly impacts the outcome of host-microbe interactions. In order to characterize novel putative acetyltransferase enzymes and their substrates, we describe a simple protocol for the detection of acetyltransferase activity in vitro. Purified proteins are incubated with 14C-acetyl CoA and separated electrophoretically, and acetylated proteins are detected by phosphorimaging or autoradiography.

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Acknowledgments

We thank Dr. Maël Baudin, Dr. Yuan Chen, and Jana Hassan for constructive feedback on the manuscript. Research on plant immunity in the Lewis laboratory was supported by the United States Department of Agriculture-Agricultural Research Service 2030-21000-046-00D (J.D.L), and NSF IOS-1557661 (J.D.L.).

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Authors and Affiliations

  1. Department of Plant and Microbial Biology, University of California—Berkeley, Berkeley, CA, USA

    Karl J. Schreiber & Jennifer D. Lewis

  2. Plant Gene Expression Center, United States Department of Agriculture, Albany, CA, USA

    Jennifer D. Lewis

Authors
  1. Karl J. Schreiber
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  2. Jennifer D. Lewis
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Corresponding author

Correspondence to Jennifer D. Lewis .

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Editors and Affiliations

  1. Division of Plant Sciences, Christopher S. Bond Life Sciences Center, and Interdisciplinary Plant Group, University of Missouri, Columbia, MO, USA

    Walter Gassmann

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Schreiber, K.J., Lewis, J.D. (2019). Protein Acetylation in Pathogen Virulence and Host Defense: In Vitro Detection of Protein Acetylation by Radiolabeled Acetyl Coenzyme A. In: Gassmann, W. (eds) Plant Innate Immunity. Methods in Molecular Biology, vol 1991. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9458-8_3

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  • DOI: https://doi.org/10.1007/978-1-4939-9458-8_3

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9457-1

  • Online ISBN: 978-1-4939-9458-8

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