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Titration of Mass Cytometry Reagents

  • Caryn van Vreden
  • Paula Niewold
  • Helen M. McGuire
  • Barbara Fazekas de St. GrothEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1989)

Abstract

Mass cytometry (MC) is a powerful research tool enabling high-dimensional analysis of single cells in suspension and within tissue sections following laser ablation. Here we describe the procedure of titrating metal-conjugated antibodies, to ensure that optimal levels of staining are achieved while minimizing nonspecific signals that may occur at high concentrations.

Key words

Mass cytometry CyTOF Helios Antibody titration Conjugation Best practices 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Caryn van Vreden
    • 1
    • 2
    • 3
  • Paula Niewold
    • 3
    • 4
  • Helen M. McGuire
    • 5
    • 6
    • 7
  • Barbara Fazekas de St. Groth
    • 2
    • 3
    • 4
    Email author
  1. 1.Sydney CytometryThe University of Sydney and Centenary InstituteSydneyAustralia
  2. 2.Ramaciotti Facility for Human Systems BiologyThe University of SydneySydneyAustralia
  3. 3.Charles Perkins CentreThe University of SydneySydneyAustralia
  4. 4.Discipline of Pathology, School of Medical Sciences, Faculty of Medicine and HealthThe University of SydneySydneyAustralia
  5. 5.Ramaciotti Facility for Human Systems BiologyThe University of SydneySydneyAustralia
  6. 6.Charles Perkins CentreThe University of SydneySydneyAustralia
  7. 7.Discipline of PathologyThe University of SydneyCamperdownAustralia

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