We wish to thank Dr. Paula Niewold for critical reading of this manuscript, and assistance with many of the experiments that lead to the development of this protocol, and to Rebecca Burrell for assistance with experiments. We would like to thank Dr. Philip Hansbro, Dr. Nicole Hansbro, Dr. Markus Hofer, David Jung, Tamara Suprunenko, Dr. Iain Campbell, and Vickie Xie for provision of bone marrow samples from various disease models over the years. We would like thank Dr. Fabian Held for assistance in troubleshooting our tSNEplots script, as the use of such a tool expedited our analysis of bone marrow data. We would also like to express gratitude to Dr. Sebastian Stifter, Dr. Carl Feng, Dr. Ben Roediger, Dr. Sean Bendall, Dr. Greg Behbehani, Dr. Helen Goodridge, and Dr. Alberto Yáñez for discussions on the nature of bone marrow hematopoiesis over the years, as these discussions have been invaluable. We would like to thank the Sydney Cytometry Facility, Ramaciotti Facility for Human System Biology, and Caryn van Vreden for assistance with mass cytometry and provision of reagents.
Thomas Ashhurst was supported by an Australian Postgraduate Award (APA) during the early development of parts of this protocol, and is currently supported by the International Society for the Advancement of Cytometry (ISAC) Marylou Ingram Scholars program. Darren Cox was also supported by an APA. This work was funded by National Health and Medical Research Council (NHMRC) funding.