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Molecular Evolution Bioinformatics Toward Structural Biology of TRPV1-4 Channels

Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 1987)

Abstract

Bioinformatics is a very resourceful tool to understand evolution of membrane proteins, such as transient receptor potential channels. Expert bioinformatics users rely on specialized scripting and programming skills. Several web servers and standalone tools are available for nonadvanced users willing to develop projects to understand their system of choice. In this case, we present a desktop-based protocol to develop evostructural hypotheses based on basic bioinformatics skills and resources, specifically for a small subgroup of TRPV channels, which can be further implemented for larger datasets.

Key words

TRP channels Evolutionary analysis Evostructural studies Computational structural biology Membrane proteins Phylogeny Sequence–structure relationships 
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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Unitat de Biofísica, Departament de Bioquímica i de Biologia Molecular, Facultat de MedicinaUniversitat Autònoma de BarcelonaBellaterraSpain
  2. 2.Institute of Adaptive and Neural Computation, School of InformaticsUniversity of EdinburghEdinburghUK

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