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Protein Acetylation pp 191–206Cite as

Biochemical and Cellular Assays to Assess the Effects of Acetylation on Base Excision Repair Enzymes

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1983))

Abstract

Protein posttranslational modifications (PTMs), including acetylation, have emerged as important regulators for controlling many cellular processes. DNA base excision repair (BER), a highly coordinated multistep cellular process, is primarily involved in the repair of both endogenous and drug-induced exogenous DNA base damages. BER relies on sequential recruitment and coordinated actions of multiple proteins. Increasing evidence suggests that acetylation of lysine residues of BER proteins facilitates fine-tuning of enzymatic activities, protein-protein interactions, and coordination of the steps in BER pathway. In this chapter, we describe detailed in vitro and in vivo approaches to examine the effect of acetylation on BER enzymes, focusing on the impact of acetylation of AP-endonuclease (APE1), a key enzyme in BER pathway, on its DNA damage repair activity, substrate-binding, and subcellular localization.

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Acknowledgment

This research was supported by NIH/NCI R01CA148941 and Nebraska state DHS LB506 grants.

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Authors and Affiliations

  1. Department of Genetics, Cell Biology and Anatomy, University of Nebraska Medical Center, Omaha, NE, USA

    Shrabasti Roychoudhury, Suravi Pramanik, Hannah L. Harris & Kishor K. Bhakat

  2. Fred and Pamela Buffet Cancer Center, University of Nebraska Medical Center, Omaha, NE, USA

    Kishor K. Bhakat

Authors
  1. Shrabasti Roychoudhury
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  2. Suravi Pramanik
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  3. Hannah L. Harris
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  4. Kishor K. Bhakat
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Corresponding author

Correspondence to Kishor K. Bhakat .

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Editors and Affiliations

  1. NIH Biomedical Research Center, National Institute on Aging, National Institutes of Health, Baltimore, MD, USA

    Robert M. Brosh, Jr.

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Roychoudhury, S., Pramanik, S., Harris, H.L., Bhakat, K.K. (2019). Biochemical and Cellular Assays to Assess the Effects of Acetylation on Base Excision Repair Enzymes. In: Brosh, Jr., R. (eds) Protein Acetylation. Methods in Molecular Biology, vol 1983. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9434-2_11

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  • DOI: https://doi.org/10.1007/978-1-4939-9434-2_11

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9433-5

  • Online ISBN: 978-1-4939-9434-2

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