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Analysis of Radiation-Induced Chromosome Exchanges Using Combinatorial Chromosome Painting

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Radiation Cytogenetics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1984))

Abstract

Combinatorial chromosome painting techniques such as multiplex fluorescence in situ hybridization (mFISH) or Spectral Karyotyping (SKY) follow basic fluorescence in situ hybridization (FISH) procedures but use combinations of fluorochromes to label probes to specific chromosomes in such a way that each chromosome is painted with a unique signal. Such signals are captured with image analysis systems allowing the construction of karyotypes with each chromosome unambiguously identified. These systems allow chromosomal analysis in great detail and are particularly useful for the detection of complex chromosome exchanges that originate from three or more breaks. This chapter will describe methods that can be used to analyze the results obtained in mFISH karyotypes particularly with relation to complex chromosome exchanges.

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Author information

Authors and Affiliations

  1. Department of Radiation Oncology, The University of Texas Medical Branch, Galveston, TX, USA

    Bradford D. Loucas

Authors
  1. Bradford D. Loucas
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Correspondence to Bradford D. Loucas .

Editor information

Editors and Affiliations

  1. Department of Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, CO, USA

    Takamitsu A. Kato

  2. Department of Radiation Oncology, University of California Davis, Sacramento, CA, USA

    Paul F. Wilson

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Loucas, B.D. (2019). Analysis of Radiation-Induced Chromosome Exchanges Using Combinatorial Chromosome Painting. In: Kato, T., Wilson, P. (eds) Radiation Cytogenetics. Methods in Molecular Biology, vol 1984. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9432-8_15

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  • DOI: https://doi.org/10.1007/978-1-4939-9432-8_15

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9430-4

  • Online ISBN: 978-1-4939-9432-8

  • eBook Packages: Springer Protocols

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