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Single-Cell RNA Sequencing with Drop-Seq

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Single Cell Methods

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1979))


Drop-Seq is a low-cost, high-throughput platform to profile thousands of cells by encapsualting them into individual droplets. Uniquely barcoded mRNA capture microparticles and cells are coconfined through a microfluidic device within the droplets where they undergo cell lysis and RNA hybridiztion. After breaking the droplets and pooling the hybridized particles, reverse transcription, PCR, and sequencing in single reactions allow to generate data from thousands of single-cell transcriptomes while maintaining information on the cellular origin of each transcript.

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J.B. was supported by a research stipend from the Fritz Thyssen Foundation. G.R. was supported by the Intramural Research Program of the Division of Intramural Research Z01AI000947, NIAID, NIH; the UCLA-Caltech MSTP, and the NIGMS T32 GM008042.

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Correspondence to Josephine Bageritz .

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Bageritz, J., Raddi, G. (2019). Single-Cell RNA Sequencing with Drop-Seq. In: Proserpio, V. (eds) Single Cell Methods. Methods in Molecular Biology, vol 1979. Humana, New York, NY.

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9239-3

  • Online ISBN: 978-1-4939-9240-9

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