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Single Cell Methods pp 9–21Cite as

Tissue Handling and Dissociation for Single-Cell RNA-Seq

Part of the Methods in Molecular Biology book series (MIMB,volume 1979)

Abstract

The starting material for all single-cell protocols is a cell suspension. The particular functions and spatial distribution of immune cells generally make them easy to isolate them from the tissues where they dwell. Here we describe tissue dissociation protocols that have been used to obtain human immune cells from lymphoid and nonlymphoid tissues to be then used as input to single-cell methods. We highlight the main factors that can influence the final quality of single-cell data, namely the stress signatures that can bias its interpretation.

Key words

  • Single-cell RNA sequencing
  • Tissue processing
  • Digestion
  • Single-cell suspension
  • Immune cells

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  • DOI: 10.1007/978-1-4939-9240-9_2
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References

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Author information

Authors and Affiliations

  1. Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, UK

    Felipe A. Vieira Braga & Ricardo J. Miragaia

Authors
  1. Felipe A. Vieira Braga
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  2. Ricardo J. Miragaia
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Corresponding author

Correspondence to Felipe A. Vieira Braga .

Editor information

Editors and Affiliations

  1. Department of Life Sciences and System Biology, University of Turin, Italian Institute for Genomic Medicine, IIGM Turin, Torino, Italy

    Valentina Proserpio

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Vieira Braga, F.A., Miragaia, R.J. (2019). Tissue Handling and Dissociation for Single-Cell RNA-Seq. In: Proserpio, V. (eds) Single Cell Methods. Methods in Molecular Biology, vol 1979. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9240-9_2

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  • DOI: https://doi.org/10.1007/978-1-4939-9240-9_2

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9239-3

  • Online ISBN: 978-1-4939-9240-9

  • eBook Packages: Springer Protocols