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Mass Spectrometry of Proteins pp 35–42Cite as

Quantitative Phosphoproteomic Using Titanium Dioxide Micro-Columns and Label-Free Quantitation

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1977))

Abstract

Phosphorylation events are important during cellular function. Analysis of phosphorylation in complex samples has been extensively studied using large-scale phosphopeptide enrichment methods. Quantitative analysis of the enriched phosphopeptides is subsequently performed using label-based methodologies (e.g., SILAC, iTRAQ, and others). Here we describe the protocol for the quantitative analysis of phosphopeptides, enriched with titanium dioxide micro-column, using an intensity-based label-free quantitation.

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Author information

Authors and Affiliations

  1. Proteomics and Mass Spectrometry, Bioscience Core Labs, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia

    Martin E. Barrios-Llerena

  2. Synthetic and Systems Biology, School of Biological Sciences, University of Edinburgh, Edinburgh, UK

    Thierry Le Bihan

Authors
  1. Martin E. Barrios-Llerena
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  2. Thierry Le Bihan
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Corresponding author

Correspondence to Martin E. Barrios-Llerena .

Editor information

Editors and Affiliations

  1. Department of Chemical and Biological Engineering, University of Sheffield, Sheffield, South Yorkshire, UK

    Caroline A. Evans

  2. Faculty of Science, Agriculture & Engineering, Newcastle University, Newcastle upon Tyne, UK

    Phillip C. Wright

  3. Laboratoire GBCM (EA4627), Conservatoire National des Arts et Métiers, HESAM Université, Paris, France

    Josselin Noirel

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Barrios-Llerena, M.E., Le Bihan, T. (2019). Quantitative Phosphoproteomic Using Titanium Dioxide Micro-Columns and Label-Free Quantitation. In: Evans, C., Wright, P., Noirel, J. (eds) Mass Spectrometry of Proteins. Methods in Molecular Biology, vol 1977. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9232-4_4

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  • DOI: https://doi.org/10.1007/978-1-4939-9232-4_4

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9231-7

  • Online ISBN: 978-1-4939-9232-4

  • eBook Packages: Springer Protocols

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