Abstract
Sampling and analysis of cerebrospinal fluid (CSF) is a common clinical practice used in the diagnosis, treatment, and prevention of neurological diseases. A similar interest is the sampling of CSF from rats to bridge the gap between bench-to-bedside work and to foster the development of new CSF biomarkers for clinical use. Here, we describe an improved procedure with an instrument designed in-house, by which rat CSF was successfully collected with indiscernible blood contamination (via the naked eye/surgical microscope amplification). The sampled CSF amounts were over 100 μl regardless of the animal’s body weight, hydration status, and symptoms of systemic damage including, but not limited to, seizure, delusion (such as repeated hemorrhagic self-biting), hematuria, and gastrointestinal bleeding. In adult Sprague-Dawley rats above 300 g, the sampled CSF amounts were reliably at 200 μl or above with this method. There were no deaths related to the CSF sampling procedure. In conclusion, the present method provides a reliable and reproducible approach for collecting 200 μl CSF in rats without blood contamination.
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Acknowledgments
This work was supported by the National Center for Toxicological Research/Food and Drug Administration [Protocol # E0758001 to M.G.P. and S.Z.I]. The authors are grateful for the technical expertise provided by the animal care staff of the Priority One Corporation and technical support provided by Susan Lantz and Bonnie Robinson.
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This document has been reviewed in accordance with the US Food and Drug Administration (FDA) policy and approved for publication. Approval does not signify that the contents necessarily reflect the position or opinions of the FDA nor does mention of trade names or commercial products constitute endorsement or recommendation for use. The findings and conclusions in this report are those of the authors and do not necessarily represent the views of the FDA.
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He, Z. et al. (2019). A Method for Sampling Rat Cerebrospinal Fluid with Minimal Blood Contamination: A Critical Tool for Biomarker Studies. In: Aschner, M., Costa, L. (eds) Cell Culture Techniques. Neuromethods, vol 145. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9228-7_12
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DOI: https://doi.org/10.1007/978-1-4939-9228-7_12
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-4939-9228-7
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