Biodrug Suppresses Breast and Colorectal Cancer in Murine Models

  • Syed Sultan Beevi
  • Naveen Kumar Tangudu
  • Vinod Kumar Verma
  • Lekha Dinesh KumarEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1974)


RNA interference (RNAi) remains one of the most promising and emerging strategies for the effective cancer treatment due to its high target specificity and greater potency. However, it is hindered due to lack of appropriate targeting technologies. Therefore, there is an imminent need to develop specific and robust delivery systems for successful gene silencing. Nanotechnology-based strategies have been in place to combat the shortcomings associated with viral-based delivery systems. Herein we describe protocols for successful in vitro and in vivo delivery of gene-specific nucleic acids such as siRNAs and shRNAs using PEI-PGMA nanoparticles for efficient cancer therapy.


RNA interference Gene silencing Nanoparticles Cancer therapy 



We acknowledge all authors of original publication for their respective contribution to the original manuscript from where this protocol is derived.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Syed Sultan Beevi
    • 1
    • 2
  • Naveen Kumar Tangudu
    • 1
    • 3
  • Vinod Kumar Verma
    • 1
    • 2
  • Lekha Dinesh Kumar
    • 1
    Email author
  1. 1.Cancer BiologyCSIR-Centre for Cellular and Molecular BiologyHyderabadIndia
  2. 2.Department of Cancer Biology, KIMS Foundation & Research CentreKIMS HospitalSecunderabadIndia
  3. 3.Institute of Comparative Molecular EndocrinologyUlm UniversityUlmGermany

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