Leishmania pp 351-368 | Cite as

Establishment, Maintenance of Phlebotomus spp. in the Laboratory, and Infection with Leishmania spp.

  • Ifhem Chelbi
  • Elyes Zhioua
Part of the Methods in Molecular Biology book series (MIMB, volume 1971)


Sand fly colonies are of major importance for experimental studies on biology, behavior, vector competence, relationship with Leishmania parasites, and vector control. This chapter is intended to provide methods and techniques used to initiate, establish, and maintain sand fly colonies. Details on collecting sand flies for colonization, colony initiation, maintenance, and experimental infection of Phlebotomus spp. with Leishmania spp. are reported.

Key words

Phlebotomus Colonization Leishmania infection 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Ifhem Chelbi
    • 1
  • Elyes Zhioua
    • 1
  1. 1.Unit of Vector EcologyInstitut Pasteur de TunisTunisTunisia

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