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Leishmania pp 225–235Cite as

DiCre-Based Inducible Gene Expression

Part of the Methods in Molecular Biology book series (MIMB,volume 1971)

Abstract

Induction of gene expression is a valuable approach for functional studies since it allows for the assessment of phenotypes without the need for clonal selection. Inducible expression can find a wide range of applications, from the study of essential genes to the characterization of overexpression of genes of interest. Here, we describe a detailed protocol for the use of the DiCre-based inducible gene expression system in Leishmania parasites. This is a tightly regulated induction system that allows for time- and dose-controlled expression of gene products, as rapidly as within 12 h.

Key words

  • Dimerizable cre recombinase
  • DiCre
  • Inducible gene expression
  • Conditional genome engineering
  • Leishmania
  • Trypanosomatids

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  • DOI: 10.1007/978-1-4939-9210-2_11
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Correspondence to Jeziel D. Damasceno .

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Damasceno, J.D., Tosi, L.R.O., Santos, R.E.R.S., Mottram, J.C. (2019). DiCre-Based Inducible Gene Expression. In: Clos, J. (eds) Leishmania. Methods in Molecular Biology, vol 1971. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9210-2_11

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  • DOI: https://doi.org/10.1007/978-1-4939-9210-2_11

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9209-6

  • Online ISBN: 978-1-4939-9210-2

  • eBook Packages: Springer Protocols