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High-Resolution and Super-Resolution Immunofluorescent Microscopy Ex Vivo to Study Pneumococcal Interactions with the Host

Part of the Methods in Molecular Biology book series (MIMB,volume 1968)

Abstract

In vivo imaging, meaning imaging tissues in living animals, is still a developing technique. However, microscopy imaging ex vivo remains a very important tool that allows for visualization of biological and pathological processes occurring in vivo. As described in Chap. 5, imaging of animal and human tissue postmortem can be performed at high resolution. Recently, imaging of human tissues infected with pneumococci using an even higher resolution, the so-called super-resolution with STED, has been reported.

Key words

  • High-Resolution microscopy
  • Super-Resolution
  • Mouse tissue
  • Human tissue
  • Immunofluorescence

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References

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Correspondence to Federico Iovino .

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Iovino, F., Henriques-Normark, B. (2019). High-Resolution and Super-Resolution Immunofluorescent Microscopy Ex Vivo to Study Pneumococcal Interactions with the Host. In: Iovino, F. (eds) Streptococcus pneumoniae. Methods in Molecular Biology, vol 1968. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9199-0_5

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  • DOI: https://doi.org/10.1007/978-1-4939-9199-0_5

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9198-3

  • Online ISBN: 978-1-4939-9199-0

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