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Construction of Fluorescent Pneumococci for In Vivo Imaging and Labeling of the Chromosome

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Streptococcus pneumoniae

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1968))

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Abstract

Advances in fluorescence imaging techniques and development and optimization of fluorescent proteins recent years have made major impacts on different fields of pneumococcal research. This chapter provides methodology for construction of fluorescent pneumococcal strains using fusions to DNA-binding proteins. By expressing fluorescent proteins fused to HlpA, a pneumococcal nucleoid binding protein, brightly fluorescent pneumococci are generated. HlpA fusions may be used both for in vivo imaging of pneumococci as well as for marking the nucleoid in cell biology studies. Furthermore, it also explains how to construct strains for imaging of specific chromosomal loci in pneumococci, using a heterologous ParBS system.

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Author information

Authors and Affiliations

  1. Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Ås, Norway

    Morten Kjos

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  1. Morten Kjos
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Correspondence to Morten Kjos .

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Editors and Affiliations

  1. Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Bioclinicum, Stockholm, Sweden

    Federico Iovino

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Kjos, M. (2019). Construction of Fluorescent Pneumococci for In Vivo Imaging and Labeling of the Chromosome. In: Iovino, F. (eds) Streptococcus pneumoniae. Methods in Molecular Biology, vol 1968. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9199-0_4

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  • DOI: https://doi.org/10.1007/978-1-4939-9199-0_4

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9198-3

  • Online ISBN: 978-1-4939-9199-0

  • eBook Packages: Springer Protocols

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