Advertisement

Bone Marrow-Derived Dendritic Cells

  • Kelly RoneyEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1960)

Abstract

Dendritic cells are a specialized type of antigen-presenting cell that bridges both innate and adaptive immune system function. While much is understood about dendritic cells and their role in the immune system, the study of these cells is critical to gain a more complete understanding of their function. The isolation and culture of dendritic cells from mouse tissues can be challenging, due in part to the low number of cells isolated. The following protocol outlines methods to optimize the isolation and culture of large numbers of dendritic cells from mouse bone marrow to facilitate a broad range of downstream experimental applications.

Keywords

Dendritic cell Mouse Bone marrow GM-CSF IL-4 

References

  1. 1.
    Altfeld M, Fadda L, Frleta D et al (2011) DCs and NK cells: critical effectors in the immune response to HIV-1. Nat Rev Immunol 11:176–186CrossRefGoogle Scholar
  2. 2.
    Bousso P (2008) T-cell activation by dendritic cells in the lymph node: lessons from the movies. Nat Rev Immunol 8:675–684CrossRefGoogle Scholar
  3. 3.
    Roy RM, Klein BS (2012) Dendritic cells in antifungal immunity and vaccine design. Cell Host Microbe 11:436–446CrossRefGoogle Scholar
  4. 4.
    Inaba K, Inaba M, Romani N et al (1992) Generation of large numbers of dendritic cells from mouse bone marrow cultures supplemented with granulocyte/macrophage colony-stimulating factor. J Exp Med 176:1693–1702CrossRefGoogle Scholar
  5. 5.
    Lutz MB, Kukutsch N, Ogilvie AL et al (1999) An advanced culture method for generating large quantities of highly pure dendritic cells from mouse bone marrow. J Immunol Methods 223:77–92CrossRefGoogle Scholar
  6. 6.
    Van Deventer HW, Serody JS, Mckinnon KP et al (2002) Transfection of macrophage inflammatory protein 1 alpha into B16 F10 melanoma cells inhibits growth of pulmonary metastases but not subcutaneous tumors. J Immunol 169:1634–1639CrossRefGoogle Scholar
  7. 7.
    Wong AW, Brickey WJ, Taxman DJ et al (2003) CIITA-regulated plexin-A1 affects T-cell-dendritic cell interactions. Nat Immunol 4:891–898CrossRefGoogle Scholar
  8. 8.
    Lutz MB, Rossner S (2007) Factors influencing the generation of murine dendritic cells from bone marrow: the special role of fetal calf serum. Immunobiology 212:855–862CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.RhoChapel HillUSA

Personalised recommendations