Toxoplasma gondii as a Model of In Vivo Host-Parasite Interactions
Toxoplasma gondii is an intracellular, apicomplexan parasite of great importance in both human and animal health. Current research has identified a variety of important and necessary factors specific to the parasite that enable it to infect and persist in a wide array of mammalian hosts. However, in order to continue to build our understanding of T. gondii pathogenesis, the relevance of these parasite characteristics needs continued investigation in animal models. In the following chapter, we present a model of intraperitoneal infection of C57BL/6 mice with T. gondii tachyzoites that, in C57BL/6 mice, elicits a strong acute immune response. Moreover, we present methods for sampling and analyzing peritoneal and bronchoalveolar lavage fluids in order to assess localized and systemic immune reactions to the parasite.
KeywordsMouse models Toxoplasma gondii Toxoplasmosis Lung harvest Cyst Tachyzoite Inflammation Peritoneal lavage Bronchoalveolar lavage Cytokine Immune system
- 10.Gorfu G, Cirelli KM, Melo MB et al (2014) Dual role for inflammasome sensors NLRP1 and NLRP3 in murine resistance to toxoplasma gondii. mBio 5(1). https://doi.org/10.1128/mBio.01117-13