Abstract
Lymphocytes are an important component of inflammatory bowel disease (IBD) pathogenesis. T cells in particular are potent inducers of inflammation in this disease as well as recurrent players in chronic lesion formation. Here we describe a method for adoptive transfer colitis in mice, which serves as a T cell-based model of inflammatory bowel disease. Adoptive transfer utilizes naïve CD4+ T cells that are administered to immunodeficient mice, which then induce a chronic pancolitis. Here, protocols are provided for the isolation and purification of naïve CD4+ T cells, as well as proper administration to test mice and important points to consider in monitoring disease progression and potential downstream applications. This method provides a way to target the contribution of T cells to IBD models, as well as providing a repeatable and physiologically relevant model of disease.
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Eden, K. (2019). Adoptive Transfer Colitis. In: Allen, I. (eds) Mouse Models of Innate Immunity. Methods in Molecular Biology, vol 1960. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9167-9_18
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DOI: https://doi.org/10.1007/978-1-4939-9167-9_18
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-9166-2
Online ISBN: 978-1-4939-9167-9
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