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Assessment of Survival and Replication of Brucella spp. in Murine Peritoneal Macrophages

  • Clayton C. CaswellEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1960)

Abstract

Brucella spp. are bacteria that naturally infect a variety of domesticated and wild animals leading to abortions and infertility, and these bacteria are also capable of causing debilitating human infections, which often result from human exposure to infected animals and animal products. The brucellae are intracellular pathogens that reside in host cells, including macrophages and dendritic cells, and it is paramount for the pathogenesis of Brucella that the bacteria are able to survive and replicate in these host cells. The methods outlined in this chapter can be employed to study the interactions between Brucella strains and primary murine peritoneal macrophages.

Keywords

Brucella Macrophage Intracellular Infection Survival Replication 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Biomedical Sciences and Pathobiology, Center for One Health Research, Virginia Maryland College of Veterinary MedicineVirginia TechBlacksburgUSA

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