Using Klebsiella pneumoniae to Model Acute Lung Inflammation in Mice

  • Dylan K. McDaniel
  • Irving C. AllenEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1960)


Lung infections caused by bacteria can induce a spectrum of immune responses, which is in part determined by the level of exposure and the degree of the host response. The host response involves pattern recognition receptors (PRRs) which sense pathogen and damage associated molecular patterns. Therefore, models of acute lung inflammation are necessary for further understanding the role of the innate immune system during bacterial infection in humans. Mice are a widely used model organism for studying important aspects of human lung pathogenesis, including acute and chronic inflammatory diseases. Klebsiella pneumoniae is a gram-negative bacterium that is commonly associated with respiratory infections, especially in a hospital setting. In this protocol, we describe a model of bacteria-mediated lung inflammation using K. pneumoniae. After a single intratracheal administration of K. pneumoniae, mice showed a strong level of Th1-mediated immune activation in the lungs. The model described here, while optimized for K. pneumonia, can be performed using other bacteria, fungi, and viruses as well.


Klebsiella pneumoniae Lung inflammation Pneumonia Pulmonary infection Macrophage Neutrophil Gram-negative bacteria 


  1. 1.
    Martin RM, Bachman MA (2018) Colonization, infection, and the accessory genome of Klebsiella pneumoniae. Front Cell Infect Microbiol 8:4. Scholar
  2. 2.
    Magill SS, Edwards JR, Bamberg W, Emerging Infections Program Healthcare-Associated I, Antimicrobial Use Prevalence Survey T et al (2014) Multistate point-prevalence survey of health care-associated infections. N Engl J Med 370(13):1198–1208. Scholar
  3. 3.
    Keynan Y, Rubinstein E (2007) The changing face of Klebsiella pneumoniae infections in the community. Int J Antimicrob Agents 30(5):385–389. Scholar
  4. 4.
    Peleg AY, Hooper DC (2010) Hospital-acquired infections due to gram-negative bacteria. N Engl J Med 362(19):1804–1813. Scholar
  5. 5.
    Zou B, Jiang W, Han H et al (2017) Acyloxyacyl hydrolase promotes the resolution of lipopolysaccharide-induced acute lung injury. PLoS Pathog 13(6):e1006436. Scholar
  6. 6.
    Willingham SB, Allen IC, Bergstralh DT et al (2009) NLRP3 (NALP3, Cryopyrin) facilitates in vivo caspase-1 activation, necrosis, and HMGB1 release via inflammasome-dependent and -independent pathways. J Immunol 183(3):2008–2015. Scholar
  7. 7.
    Allen IC, Scull MA, Moore CB et al (2009) The NLRP3 inflammasome mediates in vivo innate immunity to influenza a virus through recognition of viral RNA. Immunity 30(4):556–565. Scholar

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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary MedicineVirginia TechBlacksburgUSA
  2. 2.Department of Biomedical SciencesVirginia-Maryland Regional CollegeBlacksburgUSA

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