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Designing Glycosyltransferase Expression Constructs for Improved Purification, Protein Yield, and Crystallization

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Bacterial Polysaccharides

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1954))

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Abstract

Glycosyltransferases in bacteria are built using only four known architectures, but this structural core is often supplemented by fusions with a wide variety of other domains, including those that help recruit them to the membrane. Structural and functional characterization of these proteins is often simplified by making a subconstruct that is better behaved in solution, and perhaps monofunctional. In this chapter we review bioinformatics tools and strategies that can be used for designing such constructs of glycosyltransferases.

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Acknowledgments

This work was funded by grants from the National Science and Engineering Research Council of Canada (NSERC) and from the Canadian Glycomics Network, National Centers of Excellence (GlycoNet) to MSK and JSL.

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Author notes

  1. Taylor Forrester and Lana El Osta contributed equally to the chapter and should be considered joint first authors.

Authors and Affiliations

  1. Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON, Canada

    Taylor J. B. Forrester, Lana El Osta, Joseph S. Lam & Matthew S. Kimber

Authors
  1. Taylor J. B. Forrester
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  2. Lana El Osta
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  3. Joseph S. Lam
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  4. Matthew S. Kimber
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Corresponding author

Correspondence to Matthew S. Kimber .

Editor information

Editors and Affiliations

  1. Department of Biomedical and Molecular Sciences, Queen’s University, Kingston, ON, Canada

    Inka Brockhausen

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Forrester, T.J.B., El Osta, L., Lam, J.S., Kimber, M.S. (2019). Designing Glycosyltransferase Expression Constructs for Improved Purification, Protein Yield, and Crystallization. In: Brockhausen, I. (eds) Bacterial Polysaccharides. Methods in Molecular Biology, vol 1954. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9154-9_11

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  • DOI: https://doi.org/10.1007/978-1-4939-9154-9_11

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9153-2

  • Online ISBN: 978-1-4939-9154-9

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