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NGS-Based High-Throughput Screen to Identify MicroRNAs Regulating Growth of B-Cell Lymphoma

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Lymphoma

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1956))

Abstract

MicroRNAs (miRNAs) play important roles in development, differentiation, and homeostasis by regulating protein translation. In B-cell lymphoma, many miRNAs have altered expression levels, and for a limited subset of them, experimental data supports their functional relevance in lymphoma pathogenesis. This chapter describes an unbiased next-generation sequencing (NGS)-based high-throughput screening approach to identify miRNAs that are involved in the control of cell growth. First, we provide a protocol for performing high-throughput screening for miRNA inhibition and overexpression. Second, we describe the procedure for next-generation sequencing library preparation. Third, we provide a workflow for data analysis.

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Acknowledgments

This work was supported by grants from the National Science Centre, Poland (grant no. 2016/23/D/NZ1/01611 to A.D.-K.), and the Pediatric Oncology Foundation Groningen, the Netherlands (SKOG 11-001 to J.K. and A.v.d.B.).

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Authors and Affiliations

  1. Department of Pathology and Medical Biology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands

    Joost Kluiver, Fubiao Niu, Ye Yuan, Anke van den Berg & Agnieszka Dzikiewicz-Krawczyk

  2. Department of Clinical Pharmacy, The Second Affiliated Hospital of Harbin Medical University, Harbin, China

    Ye Yuan

  3. Department of Genetics, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands

    Klaas Kok

  4. Institute of Human Genetics, Polish Academy of Sciences, Poznan, Poland

    Agnieszka Dzikiewicz-Krawczyk

Authors
  1. Joost Kluiver
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  2. Fubiao Niu
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  3. Ye Yuan
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  4. Klaas Kok
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  5. Anke van den Berg
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  6. Agnieszka Dzikiewicz-Krawczyk
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Corresponding authors

Correspondence to Joost Kluiver or Agnieszka Dzikiewicz-Krawczyk .

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Editors and Affiliations

  1. Institute of Cell Biology (Cancer Research), Medical School, University of Duisburg-Essen, Nordrhein-Westfalen, Essen, Germany

    Ralf Küppers

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Kluiver, J., Niu, F., Yuan, Y., Kok, K., van den Berg, A., Dzikiewicz-Krawczyk, A. (2019). NGS-Based High-Throughput Screen to Identify MicroRNAs Regulating Growth of B-Cell Lymphoma. In: Küppers, R. (eds) Lymphoma. Methods in Molecular Biology, vol 1956. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9151-8_12

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  • DOI: https://doi.org/10.1007/978-1-4939-9151-8_12

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9150-1

  • Online ISBN: 978-1-4939-9151-8

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