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Isolation and Characterization of Extracellular Vesicles Derived from Trypanosoma cruzi

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T. cruzi Infection

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1955))

Abstract

Extracellular vesicles (EVs) are heterogeneous membrane-surrounded structures that participate in cellular communications, which comprise exosomes and microvesicles. These vesicles have different biogenesis, and their physiological and pathological roles in chronic and infectious diseases are under constant investigation. In Chagas disease, Trypanosoma cruzi EVs have been described using different approaches. The isolation of T. cruzi-derived EVs has been done mainly using the differential centrifugation technique, and different strategies have been employed for characterization of them. Here, we describe the method to isolate EVs by differential centrifugation and a detection protocol for EVs in T. cruzi-host cell interaction to allow further investigations about this parasite.

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Authors and Affiliations

  1. Departamento de Bioquímica, Universidade Federal do Paraná, Curitiba, PR, Brazil

    Izadora Volpato Rossi, Bruno Gavinho & Marcel Ivan Ramirez

  2. Fundação Instituto Oswaldo Cruz, Rio de Janeiro, RJ, Brazil

    Marcel Ivan Ramirez

  3. Programa de Pós-Graduação em Microbiologia, Patologia e Parasitologia da Universidade Federal do Paraná, Curitiba, PR, Brazil

    Izadora Volpato Rossi & Bruno Gavinho

Authors
  1. Izadora Volpato Rossi
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  2. Bruno Gavinho
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  3. Marcel Ivan Ramirez
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Editor information

Editors and Affiliations

  1. INGEBI-CONICET, Buenos Aires, Argentina

    Karina Andrea Gómez

  2. UNSAM-CONICET, IIB-INTECH, San Martin, Buenos Aires, Argentina

    Carlos Andrés Buscaglia

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Rossi, I.V., Gavinho, B., Ramirez, M.I. (2019). Isolation and Characterization of Extracellular Vesicles Derived from Trypanosoma cruzi. In: Gómez, K., Buscaglia, C. (eds) T. cruzi Infection. Methods in Molecular Biology, vol 1955. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9148-8_7

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  • DOI: https://doi.org/10.1007/978-1-4939-9148-8_7

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9147-1

  • Online ISBN: 978-1-4939-9148-8

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