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Methods for Natural Transformation in Acinetobacter baumannii

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Acinetobacter baumannii

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1946))

Abstract

The genomes of Acinetobacter baumannii tell us stories about horizontal gene transfer (HGT) events that steadily drive the evolution of this nosocomial pathogen toward multidrug resistance. Natural transformation competence constitutes one of the several possible pathways that mediate HGT in A. baumannii. Here, we describe and discuss the methods for studying DNA uptake in A. baumannii via natural transformation.

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Acknowledgment

G.W. acknowledges financial support from the Deutsche Forschungsgemeinschaft (DFG-FOR 2251, WI 3272/3-1).

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Authors and Affiliations

  1. Robert Koch Institute, Wernigerode, Germany

    Gottfried Wilharm & Evelyn Skiebe

Authors
  1. Gottfried Wilharm
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  2. Evelyn Skiebe
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Correspondence to Gottfried Wilharm .

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Editors and Affiliations

  1. Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS, USA

    Indranil Biswas

  2. Department of Microbiology and Immunology, Emory University, Atlanta, GA, USA

    Philip N. Rather

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Wilharm, G., Skiebe, E. (2019). Methods for Natural Transformation in Acinetobacter baumannii. In: Biswas, I., Rather, P. (eds) Acinetobacter baumannii. Methods in Molecular Biology, vol 1946. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9118-1_8

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  • DOI: https://doi.org/10.1007/978-1-4939-9118-1_8

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9117-4

  • Online ISBN: 978-1-4939-9118-1

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