Abstract
This chapter describes protocols for determining A. baumannii isolates’ overall levels of sensitivity to heavy metals; copper is used as a model heavy metal. Measurements of the ability of strains to grow in the presence of various concentrations of copper in liquid media and on copper-containing surfaces are described.
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References
Ladomersky E, Petris MJ (2015) Copper tolerance and virulence in bacteria. Metallomics 7(6):957–964. https://doi.org/10.1039/c4mt00327f
Djoko KY, Ong CL, Walker MJ, McEwan AG (2015) The role of copper and zinc toxicity in innate immune defense against bacterial pathogens. J Biol Chem 290(31):18954–18961. https://doi.org/10.1074/jbc.R115.647099
Peleg AY, Seifert H, Paterson DL (2008) Acinetobacter baumannii: emergence of a successful pathogen. Clin Microbiol Rev 21(3):538–582. https://doi.org/10.1128/CMR.00058-07
Halachev MR, Chan JZ, Constantinidou CI, Cumley N, Bradley C, Smith-Banks M, Oppenheim B, Pallen MJ (2014) Genomic epidemiology of a protracted hospital outbreak caused by multidrug-resistant Acinetobacter baumannii in Birmingham, England. Genome Med 6(11):70. https://doi.org/10.1186/s13073-014-0070-x
Schmidt MG, Attaway HH, Sharpe PA, John J Jr, Sepkowitz KA, Morgan A, Fairey SE, Singh S, Steed LL, Cantey JR, Freeman KD, Michels HT, Salgado CD (2012) Sustained reduction of microbial burden on common hospital surfaces through introduction of copper. J Clin Microbiol 50(7):2217–2223. https://doi.org/10.1128/JCM.01032-12
Salgado CD, Sepkowitz KA, John JF, Cantey JR, Attaway HH, Freeman KD, Sharpe PA, Michels HT, Schmidt MG (2013) Copper surfaces reduce the rate of healthcare-acquired infections in the intensive care unit. Infect Control Hosp Epidemiol 34(5):479–486. https://doi.org/10.1086/670207
Schmidt MG, Attaway HH, Fairey SE, Steed LL, Michels HT, Salgado CD (2013) Copper continuously limits the concentration of bacteria resident on bed rails within the intensive care unit. Infect Control Hosp Epidemiol 34(5):530–533. https://doi.org/10.1086/670224
Schmidt MG, von Dessauer B, Benavente C, Benadof D, Cifuentes P, Elgueta A, Duran C, Navarrete MS (2016) Copper surfaces are associated with significantly lower concentrations of bacteria on selected surfaces within a pediatric intensive care unit. Am J Infect Control 44(2):203–209. https://doi.org/10.1016/j.ajic.2015.09.008
von Dessauer B, Navarrete MS, Benadof D, Benavente C, Schmidt MG (2016) Potential effectiveness of copper surfaces in reducing health care-associated infection rates in a pediatric intensive and intermediate care unit: a nonrandomized controlled trial. Am J Infect Control. https://doi.org/10.1016/j.ajic.2016.03.053
Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
Warnes SL, Caves V, Keevil CW (2012) Mechanism of copper surface toxicity in Escherichia coli O157:H7 and Salmonella involves immediate membrane depolarization followed by slower rate of DNA destruction which differs from that observed for Gram-positive bacteria. Environ Microbiol 14(7):1730–1743. https://doi.org/10.1111/j.1462-2920.2011.02677.x
Warnes SL, Green SM, Michels HT, Keevil CW (2010) Biocidal efficacy of copper alloys against pathogenic enterococci involves degradation of genomic and plasmid DNAs. Appl Environ Microbiol 76(16):5390–5401. https://doi.org/10.1128/AEM.03050-09
Williams CL, Neu HM, Gilbreath JJ, Michel SL, Zurawski DV, Merrell DS (2016) Characterization of copper resistance in Acinetobacter baumannii. Appl Environ Microbiol 82(20):6174–6188. https://doi.org/10.1128/AEM.01813-16
Tipton KA, Dimitrova D, Rather PN (2015) Phase-variable control of multiple phenotypes in Acinetobacter baumannii strain AB5075. J Bacteriol 197(15):2593–2599. https://doi.org/10.1128/JB.00188-15
Eser OK, Ergin A, Hascelik G (2015) Antimicrobial activity of copper alloys against invasive multidrug-resistant nosocomial pathogens. Curr Microbiol 71(2):291–295. https://doi.org/10.1007/s00284-015-0840-8
Espirito Santo C, Taudte N, Nies DH, Grass G (2008) Contribution of copper ion resistance to survival of Escherichia coli on metallic copper surfaces. Appl Environ Microbiol 74(4):977–986. https://doi.org/10.1128/AEM.01938-07
Souli M, Galani I, Plachouras D, Panagea T, Armaganidis A, Petrikkos G, Giamarellou H (2013) Antimicrobial activity of copper surfaces against carbapenemase-producing contemporary Gram-negative clinical isolates. J Antimicrob Chemother 68(4):852–857. https://doi.org/10.1093/jac/dks473
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Disclaimer and Funding: The opinions or assertions contained herein are the private ones of the authors and are not to be construed as official or reflecting the views of the Department of Defense, the Uniformed Services University of the Health Sciences, or any other agency of the US Government.
Work in the Merrell lab is supported by funds from the NIH and DoD.
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Williams, C.L., Merrell, D.S. (2019). Testing Metal Sensitivity of A. baumannii Strains: Survival in Copper-Supplemented Liquid Media and on Copper-Containing Surfaces. In: Biswas, I., Rather, P. (eds) Acinetobacter baumannii. Methods in Molecular Biology, vol 1946. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9118-1_5
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DOI: https://doi.org/10.1007/978-1-4939-9118-1_5
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