Microbubbles for Nucleic Acid Delivery in Liver Using Mild Sonoporation
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Ultrasound-mediated gene delivery is an interesting approach, which could help in increasing gene transfer in deep tissues. Moreover, it allows for performing experiments guided by the image to determine which elements are required. Microbubbles complexed with a eukaryotic expression cassette are excellent agents as they are responsive to ultrasounds and, upon oscillation, can destabilize membranes to enhance gene transfer. Here, we describe the preparation of positively charged microbubbles, plasmid free of antibiotic resistance marker, their combination and the conditions of ultrasound-mediated liver transfection post-systemic administration in mice. This association allowed us to obtain a superior liver gene expression at least over 8 months after a single injection.
Key wordsMicrobubbles Cationic lipid Lipoplex characterization Ultrasound delivery Optical imaging Gene expression pFAR
- 3.Delalande A, Bouakaz A, Renault G, Tabareau K, Kotopoulis S, Midoux P, Arbeille B, Uzbeko R, Chakravarti S, Postema M, Pichon C (2011) Ultrasound and microbubble-assisted gene delivery in Achilles tendons: long lasting gene expression and restoration of fibromodulin KO phenotype. J Control Release 156:223–230CrossRefGoogle Scholar
- 14.Manta S, Renault G, Delalande A, Couture O, Lagoutte I, Seguin J, Lager F, Houzé P, Midoux P, Bessodes M, Scherman D, Bureau MF, Marie C, Pichon C, Mignet N (2017) Cationic microbubbles and antibiotic-free miniplasmid for sustained ultrasound-mediated transgene expression in liver. J Control Release 262:170–181CrossRefGoogle Scholar