Microbubbles for Nucleic Acid Delivery in Liver Using Mild Sonoporation

Part of the Methods in Molecular Biology book series (MIMB, volume 1943)


Ultrasound-mediated gene delivery is an interesting approach, which could help in increasing gene transfer in deep tissues. Moreover, it allows for performing experiments guided by the image to determine which elements are required. Microbubbles complexed with a eukaryotic expression cassette are excellent agents as they are responsive to ultrasounds and, upon oscillation, can destabilize membranes to enhance gene transfer. Here, we describe the preparation of positively charged microbubbles, plasmid free of antibiotic resistance marker, their combination and the conditions of ultrasound-mediated liver transfection post-systemic administration in mice. This association allowed us to obtain a superior liver gene expression at least over 8 months after a single injection.

Key words

Microbubbles Cationic lipid Lipoplex characterization Ultrasound delivery Optical imaging Gene expression pFAR 


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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Unité de Technologies Chimiques et Biologiques pour la Santé (UTCBS), INSERM, U1022ParisFrance
  2. 2.INSERM, U1022ParisFrance
  3. 3.CNRS, UMR8258ParisFrance
  4. 4.Faculté de Pharmacie, Sorbonne Paris CitéUniversité Paris DescartesParisFrance
  5. 5.Chimie ParisTechPSL Research UniversityParisFrance
  6. 6.Centre de Biophysique Moléculaire and Université d’OrléansCNRS-UPR 4301OrléansFrance
  7. 7.INSERM, U1016, Institut CochinParisFrance
  8. 8.CNRS, UMR8104ParisFrance
  9. 9.Sorbonne Paris CitéUniversité Paris DescartesParisFrance

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