Abstract
Research on lung development and disease frequently utilizes mouse models to conduct in vitro experiments. Such experiments involve multiple methodologically distinct stages, from careful consideration of mouse models used to obtain biological samples, to the culturing and imaging of those samples, and finally, to post-imaging analysis. Here, we detail basic protocols to assist with each of these stages. First, we discuss harvesting and preparing biological samples; second, we focus on culturing embryonic whole lung explants and isolated mesenchyme and epithelium; third, we specify the basics of obtaining still and live images; and finally, we bring these methods together by considering and briefly analyzing a lineage-labelling experiment.
Key words
- Lung explant
- Isolated epithelium
- Isolated mesenchyme
- Organ culture
- Tissue culture
- Lineage tracing
- Still imaging
- Live imaging
- Image analysis
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Acknowledgments
We wish to thank Salma Dilai for her contribution to the manuscript. S.B. was supported by grants from the Deutsche Forschungsgemeinschaft (DFG; BE4443/1-1, BE4443/4-1, BE4443/6-1, KFO309 P7, and SFB1213-projects A02 and A04), Landes-Offensive zur Entwicklung Wissenschaftlich-Ökonomischer Exzellenz (LOEWE), UKGM, Universities of Giessen and Marburg Lung Center (UGMLC), DZL, and COST (BM1201).
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Jones, M., Bellusci, S. (2019). Imaging and Analysis of Mouse Embryonic Whole Lung, Isolated Tissue, and Lineage-Labelled Cell Culture. In: Bertoncello, I. (eds) Mouse Cell Culture. Methods in Molecular Biology, vol 1940. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9086-3_8
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DOI: https://doi.org/10.1007/978-1-4939-9086-3_8
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