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Glutamate Receptors pp 57–64Cite as

Preparation of Organotypic Slice Cultures for the Study of Glutamate Receptor Function

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1941))

Abstract

Organotypic slice cultures enable the study of glutamate receptors in an environment closely related to the in vivo situation but with easy access to genetic manipulation of the receptors and regulatory signaling cascades as well as a more precise pharmacological intervention. We describe a method to prepare organotypic hippocampal slices that can be easily adapted to other brain regions. Brain slices are laid on porous membranes, and culture medium is allowed to form an interface. This method preserves the functionality and gross architecture of the hippocampus for up to 2 weeks in culture.

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Author information

Authors and Affiliations

  1. Department of Physiology and Biophysics, University of Washington School of Medicine, Seattle, WA, USA

    Andres Barria

Authors
  1. Andres Barria
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Corresponding author

Correspondence to Andres Barria .

Editor information

Editors and Affiliations

  1. Department of Neurology, University of Wisconsin, Madison, WI, USA

    Corinna Burger

  2. ANSER, Homeland Security Studies and Analysis Institute, Falls Church, VA, USA

    Margaret Jo Velardo

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Barria, A. (2019). Preparation of Organotypic Slice Cultures for the Study of Glutamate Receptor Function. In: Burger, C., Velardo, M. (eds) Glutamate Receptors. Methods in Molecular Biology, vol 1941. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9077-1_5

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  • DOI: https://doi.org/10.1007/978-1-4939-9077-1_5

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9076-4

  • Online ISBN: 978-1-4939-9077-1

  • eBook Packages: Springer Protocols

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