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Conditional Mutagenesis in Oligodendrocyte Lineage Cells

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Oligodendrocytes

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1936))

Abstract

Cell-type-specific gene targeting with the Cre/loxP system has become an indispensable technique in experimental neuroscience, particularly for the study of late-born glial cells that make myelin. A plethora of conditional mutants and Cre-expressing mouse lines is now available to the research community that allows laboratories to readily engage in in vivo analyses of oligodendrocytes and their precursor cells. This chapter summarizes concepts and strategies in targeting myelinating glial cells in mice for mutagenesis or imaging, and provides an overview of the most important Cre driver lines successfully used in this rapidly growing field.

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Acknowledgments

We thank Peter Brophy, Brian Popko, Dwight Bergles, Ueli Suter, Bill Richardson, Ori Peles, David Rowitch, and Richard Lu for personal communications on Cre driver lines, members of the Department of Neurogenetics for critical discussion, and Georg Wieser and Ulli Bode for help with the figures. Work in the authors’ laboratories was supported by grants from the DFG (SPP 1757 to S.G. and K.A.N.) and by an European Research Council (ERC) advanced grant (to K.A.N.).

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Authors and Affiliations

  1. Department of Neurogenetics, Max Planck Institute of Experimental Medicine, Göttingen, Germany

    Sandra Goebbels & Klaus-Armin Nave

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  1. Sandra Goebbels
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  2. Klaus-Armin Nave
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Correspondence to Sandra Goebbels or Klaus-Armin Nave .

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Editors and Affiliations

  1. Centre for Discovery Brain Sciences, University of Edinburgh, Edinburgh, UK

    David A. Lyons

  2. Centre for Discovery Brain Sciences, University of Edinburgh, Edinburgh, UK

    Linde Kegel

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Goebbels, S., Nave, KA. (2019). Conditional Mutagenesis in Oligodendrocyte Lineage Cells. In: Lyons, D., Kegel, L. (eds) Oligodendrocytes. Methods in Molecular Biology, vol 1936. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9072-6_15

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  • DOI: https://doi.org/10.1007/978-1-4939-9072-6_15

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