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Stable Genetic Modification of Mesenchymal Stromal Cells Using Lentiviral Vectors

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Book cover Viral Vectors for Gene Therapy

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1937))

Abstract

Mesenchymal stromal cell (MSC) therapy has produced very promising results for multiple diseases in animal models, with over 780 clinical trials on going or completed. However, most of the human clinical trials have not been as successful as trials using preclinical models. To improve the therapeutic potential of MSCs, different research groups have used gene transfer vectors to express factors involved in migration, survival, differentiation, and immunomodulation. The ideal gene transfer vector for most applications should achieve long-term, stable (constitutive or inducible) transgene expression in MSCs and their progeny. Given their efficiency and low impact on transduced cells, lentiviral vectors (LVs) are the vectors of choice. In this chapter we will describe a detailed protocol for the generation of genetically modified MSCs using lentiviral vectors (LVs). Although this protocol has been optimized for MSC lentiviral transduction, it can be easily adapted to other stem cells by changing culture conditions while maintaining volumes and incubation times.

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Authors and Affiliations

  1. Centre for Genomics and Oncological Research (GENYO), Pfizer/University of Granada/Andalusian Regional Government, PTS Granada, Granada, Spain

    Francisco Martín, María Tristán-Manzano, Noelia Maldonado-Pérez, Sabina Sánchez-Hernández, Karim Benabdellah & Marién Cobo

Authors
  1. Francisco Martín
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  2. María Tristán-Manzano
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  3. Noelia Maldonado-Pérez
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  4. Sabina Sánchez-Hernández
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  5. Karim Benabdellah
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  6. Marién Cobo
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Corresponding author

Correspondence to Francisco Martín .

Editor information

Editors and Affiliations

  1. Translational Science and Molecular Medicine, Michigan State University, Grand Rapids, MI, USA

    Fredric P. Manfredsson

  2. Translational Science and Molecular Medicine, Michigan State University, Grand Rapids, MI, USA

    Matthew J. Benskey

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Martín, F., Tristán-Manzano, M., Maldonado-Pérez, N., Sánchez-Hernández, S., Benabdellah, K., Cobo, M. (2019). Stable Genetic Modification of Mesenchymal Stromal Cells Using Lentiviral Vectors. In: Manfredsson, F., Benskey, M. (eds) Viral Vectors for Gene Therapy. Methods in Molecular Biology, vol 1937. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9065-8_17

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  • DOI: https://doi.org/10.1007/978-1-4939-9065-8_17

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9064-1

  • Online ISBN: 978-1-4939-9065-8

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