Abstract
Inner ear gene therapy offers great potential as a treatment for hearing loss and dizziness. The surgical method used to deliver gene therapy into the inner ear is a critical step in determining the success of inner ear gene therapy. Here we describe two commonly used surgical methods for gene delivery in neonatal mouse inner ear: the round window approach and the posterior semicircular canal approach. Both of these approaches are effective at delivering gene therapy to the neonatal mouse inner ear.
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Acknowledgment
This work was supported by funds from the NIDCD Division of Intramural Research/NIH (DC000082-02 to W.W.C., as well as DC000081 to advanced imaging core). We are grateful for the NIDCD animal facility staff for caring for our animals. We would like to thank Dr. Lisa Cunningham and Dr. Nicole Schmitt for critiquing the manuscript.
Disclosures: The authors have no conflict of interest to disclose.
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Isgrig, K., Chien, W.W. (2019). Surgical Methods for Inner Ear Gene Delivery in Neonatal Mouse. In: Manfredsson, F., Benskey, M. (eds) Viral Vectors for Gene Therapy. Methods in Molecular Biology, vol 1937. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9065-8_13
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DOI: https://doi.org/10.1007/978-1-4939-9065-8_13
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-9065-8
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