Abstract
The analysis of the lung microbiome composition is a field of research that recently emerged. It gained great interest in pulmonary diseases such as pneumonia since the microbiome seems to be involved in host immune responses, inflammation, and protection against pathogens. Thus, it is possible that the microbial communities living in the lungs play a role in the outcome and severity of lung infections such as Legionella-caused pneumonia and in the response to antibiotic therapy. In this chapter, all steps necessary for the characterization of the bacterial and fungal fraction of the lung microbiome using high-throughput sequencing approaches are explained, starting from the selection of clinical samples to the analysis of the taxonomic composition, diversity, and ecology of the microbiome.
Key words
- Pulmonary microbiome
- Legionella pneumophila
- 16S rRNA gene
- ITS
- High-throughput sequencing
- Bioinformatics
- Microbial ecology
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Acknowledgments
Work in the CB laboratory is financed by the Institut Pasteur and AECP was fianced by a fellowship from grant ANR-10-LABX-62-IBEID and grant ANR 15 CE17 0014 03.
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Pérez-Cobas, A.E., Buchrieser, C. (2019). Analysis of the Pulmonary Microbiome Composition of Legionella pneumophila-Infected Patients. In: Buchrieser, C., Hilbi, H. (eds) Legionella. Methods in Molecular Biology, vol 1921. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9048-1_27
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DOI: https://doi.org/10.1007/978-1-4939-9048-1_27
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