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Legionella pp 241–247Cite as

Isolation of the Dot/Icm Type IV Secretion System Core Complex from Legionella pneumophila

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1921))

Abstract

Legionella utilizes specialized protein secretion machinery called the type IV secretion system encoded by dot/icm genes to modulate host cellular systems. We describe here the procedure to isolate the core complex of the Dot/Icm type IV secretion system of L. pneumophila based on detergent lysis of bacteria and ultracentrifugation. The isolated protein complex can be applied for biochemical and transmission electron microscopy analysis.

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References

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Acknowledgment

This work has been financially supported by MEXT/JSPS KAKENHI Grants 15H01322 (to TK). The protocol presented here has been adapted from Kubori et al. [5].

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Authors and Affiliations

  1. Department of Microbiology, Graduate School of Medicine, Gifu University, Gifu, Japan

    Tomoko Kubori & Hiroki Nagai

Authors
  1. Tomoko Kubori
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  2. Hiroki Nagai
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Corresponding author

Correspondence to Tomoko Kubori .

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Editors and Affiliations

  1. Institut Pasteur, Biologie des Bactéries Intracellulaires, Paris, France

    Carmen Buchrieser

  2. Institute of Medical Microbiology, University of Zürich, Zürich, Switzerland

    Hubert Hilbi

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Kubori, T., Nagai, H. (2019). Isolation of the Dot/Icm Type IV Secretion System Core Complex from Legionella pneumophila. In: Buchrieser, C., Hilbi, H. (eds) Legionella. Methods in Molecular Biology, vol 1921. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9048-1_15

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  • DOI: https://doi.org/10.1007/978-1-4939-9048-1_15

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9047-4

  • Online ISBN: 978-1-4939-9048-1

  • eBook Packages: Springer Protocols

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