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Transcriptome-Wide Mapping 5-Methylcytosine by m5C RNA Immunoprecipitation Followed by Deep Sequencing in Plant

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Plant Long Non-Coding RNAs

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1933))

Abstract

Transcriptome-wide mapping RNA modification is crucial to understand the distribution and function of RNA modifications. Here, we describe a protocol to transcriptome-wide mapping 5-methylcytosine (m5C) in plant, by a RNA immunoprecipitation followed by deep sequencing (m5C-RIP-seq) approach. The procedure includes RNA extraction, fragmentation, RNA immunoprecipitation, and library construction.

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Acknowledgments

This work was supported by Ministry of Science and Technology of the People’s Republic of China (2016YFD0101001) to X.G., National Natural Science Foundation of China (31671670) to X.G., and Recruitment program of Global Youth Expert of China to X.G.

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Authors and Affiliations

  1. Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China

    Xiaofeng Gu

  2. Department of Biological Sciences, National University of Singapore, Singapore, Singapore

    Zhe Liang

Authors
  1. Xiaofeng Gu
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  2. Zhe Liang
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Corresponding author

Correspondence to Xiaofeng Gu .

Editor information

Editors and Affiliations

  1. Guangxi Key Laboratory of Sugarcane Biology, Guangxi University, Nanning, Guangxi, China

    Julia A. Chekanova  & Hsiao-Lin V. Wang  & 

  2. Department of Biology, Emory University, Atlanta, Georgia, USA

    Hsiao-Lin V. Wang

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Gu, X., Liang, Z. (2019). Transcriptome-Wide Mapping 5-Methylcytosine by m5C RNA Immunoprecipitation Followed by Deep Sequencing in Plant. In: Chekanova, J.A., Wang, HL.V. (eds) Plant Long Non-Coding RNAs. Methods in Molecular Biology, vol 1933. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9045-0_24

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  • DOI: https://doi.org/10.1007/978-1-4939-9045-0_24

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9044-3

  • Online ISBN: 978-1-4939-9045-0

  • eBook Packages: Springer Protocols

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