Abstract
Bioinformatic analysis of small RNA sequencing libraries consists of transforming a series of small RNA sequencing experiment fastq files into a table containing small RNA sequences and their abundance. This is achieved by cleaning the reads, aligning the cleaned reads to a reference, and parsing the alignment results. In this protocol we present the most common option, and the rationale, for each of these steps.
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Acknowledgments
Work in the SDF laboratory was financed by the Mexican National Council of Science and Technology (CONACyT) grants CB-2012-177739 and FC-2015-2/1061.
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Chávez Montes, R.A., Jaimes-Miranda, F., de Folter, S. (2019). Bioinformatic Analysis of Small RNA Sequencing Libraries. In: de Folter, S. (eds) Plant MicroRNAs. Methods in Molecular Biology, vol 1932. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9042-9_4
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DOI: https://doi.org/10.1007/978-1-4939-9042-9_4
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