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Three-Dimensional Structured Illumination Microscopy (3D-SIM) to Dissect Signaling Cross-Talks in Motile T-Cells

Part of the Methods in Molecular Biology book series (MIMB,volume 1930)

Abstract

Visualization of signal transduction events in T-cells has always been a challenge due to their miniscule size. Recent advancement in super-resolution microscopy techniques presents many new opportunities to navigate the spatial and temporal signaling cross-talks in motile T-cells. Here, we provide technical details, optimal conditions, and critical practical considerations that need to be taken into account during cell handling, sample preparation, and image acquisition of motile T-cells for performing three-dimensional structured illumination microscopy (3D-SIM).

Key words

  • Super-resolution microscopy
  • 3D-SIM
  • Immunostaining

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  • DOI: 10.1007/978-1-4939-9036-8_6
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Acknowledgments

This work was supported in part by grants from Lee Kong Chian School of Medicine, Nanyang Technological University Singapore Start-Up Grant and the Ministry of Education Singapore under its Singapore Ministry of Education Academic Research Fund (AcRF) Tier 2 Grant (MOE2017-T2-2-004) to N.K.V. 3D-SIM platform (DeltaVision OMX v4 Blaze microscope) and Institute of Medical Biology (IMB) Microscopy Unit, now renamed to the A*STAR Microscopy Platform within the Skin Research Institute of Singapore (SRIS), was funded by A*STAR, Singapore.

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Correspondence to Seow Theng Ong .

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Ong, S.T., Wright, G.D., Verma, N.K. (2019). Three-Dimensional Structured Illumination Microscopy (3D-SIM) to Dissect Signaling Cross-Talks in Motile T-Cells. In: Verma, N. (eds) T-Cell Motility. Methods in Molecular Biology, vol 1930. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9036-8_6

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  • DOI: https://doi.org/10.1007/978-1-4939-9036-8_6

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9035-1

  • Online ISBN: 978-1-4939-9036-8

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