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Monitoring Interactions Between S100B and the Dopamine D2 Receptor Using NMR Spectroscopy

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Calcium-Binding Proteins of the EF-Hand Superfamily

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1929))

Abstract

S100B is a dimeric EF-hand protein that undergoes a calcium-induced conformational change and interacts with a wide range of proteins to modulate their functions. The dopamine D2 receptor is one potential S100B binding partner that may play a key role in neurological processing. In this chapter, we describe the use of NMR spectroscopy to examine the interaction between calcium-bound S100B and the third intracellular loop (IC3) from the dopamine D2 receptor. We provide details that allow the strength of the interaction (K d) between the two proteins to be determined and the IC3 site of interaction on the structure of S100B to be identified. Both these characteristics can be identified from a single series of nondestructive experiments.

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Acknowledgment

This research was supported by a research grant (MOP 93520) from the Canadian Institutes of Health Research (GSS). We thank Brian Dempsey for the helpful discussions. Yuning Wang and Roya Tadayon contributed equally to this work.

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Author notes

  1. Yuning Wang and Roya Tadayon contributed equally to this work.

Authors and Affiliations

  1. Department of Biochemistry, The University of Western Ontario, London, ON, Canada

    Yuning Wang, Roya Tadayon & Gary S. Shaw

Authors
  1. Yuning Wang
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  2. Roya Tadayon
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  3. Gary S. Shaw
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Corresponding author

Correspondence to Gary S. Shaw .

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Editors and Affiliations

  1. Division of Clinical Chemistry and Biochemistry, Department of Pediatrics, University of Zürich, Zürich, Switzerland

    Claus W. Heizmann

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Wang, Y., Tadayon, R., Shaw, G.S. (2019). Monitoring Interactions Between S100B and the Dopamine D2 Receptor Using NMR Spectroscopy. In: Heizmann, C. (eds) Calcium-Binding Proteins of the EF-Hand Superfamily. Methods in Molecular Biology, vol 1929. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9030-6_20

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  • DOI: https://doi.org/10.1007/978-1-4939-9030-6_20

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9029-0

  • Online ISBN: 978-1-4939-9030-6

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