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Cancer Metabolism pp 409–426Cite as

Hyperpolarized MRI for Studying Tumor Metabolism

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1928))

Abstract

Hyperpolarized magnetic resonance imaging (MRI) can be used to detect real-time in vivo tumor metabolism. Dissolution dynamic nuclear polarization method increases polarization of 13C-labeled molecules, typically [1-13C]pyruvate, which can be injected into an animal during MRI scanning. Increased polarization leads to a higher observed signal, which allows for the detection and imaging of the transfer of 13C-label between the injected marker molecule, pyruvate, and its metabolic products, most importantly lactate. This information can be used to assess the metabolic status of the tumor, for example, during therapy. Here, the basic methodology and data analysis for a preclinical hyperpolarized pyruvate experiment are described.

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Acknowledgments

This work was supported by the Academy of Finland grants no. 286895 and no. 314551. The author shares dDNP-related intellectual property with GE Healthcare.

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Authors and Affiliations

  1. A.I. Virtanen Institute for Molecular Sciences, University of Eastern Finland, Kuopio, Finland

    Mikko I. Kettunen

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  1. Mikko I. Kettunen
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Correspondence to Mikko I. Kettunen .

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Editors and Affiliations

  1. National Cancer Institute, North Bethesda, MD, USA

    Majda Haznadar

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Kettunen, M.I. (2019). Hyperpolarized MRI for Studying Tumor Metabolism. In: Haznadar, M. (eds) Cancer Metabolism. Methods in Molecular Biology, vol 1928. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9027-6_21

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  • DOI: https://doi.org/10.1007/978-1-4939-9027-6_21

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9026-9

  • Online ISBN: 978-1-4939-9027-6

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