Abstract
New mass spectrometry approaches enable antibody-independent tracking of protein production. Herein, we outline an antibody-independent mass spectrometry method for tracking recombinant protein production in the methylotrophic yeast Pichia pastoris system.
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Acknowledgments
This work was supported by grants from the Canadian Institutes of Health Research (CIHR, MOP#133543 to JAM), the European Commission Research Executive Agency (Marie Sklodowska-Curie International Incoming Fellowship to JAM and RMB), and the Biotechnology and Biological Sciences Research Council (International Partnering Award BB/P025927/1 to JAM and RMB).
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Bill, R.M., Ulke-Lemée, A., Cartwright, S.P., Far, R., Kim, J., MacDonald, J.A. (2019). Analyzing Recombinant Protein Production in Pichia pastoris with Targeted Proteomics. In: Gasser, B., Mattanovich, D. (eds) Recombinant Protein Production in Yeast. Methods in Molecular Biology, vol 1923. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9024-5_8
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DOI: https://doi.org/10.1007/978-1-4939-9024-5_8
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Online ISBN: 978-1-4939-9024-5
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