Abstract
To date, many mutants have been isolated from dicot plants, including Arabidopsis thaliana, and the physiological roles of the isolated genes have been identified. Molecular genetic analyses have usually been conducted in the model plant Arabidopsis to identify blue-light photoreceptors and key signaling components in phototropic responses. Despite these investigations, several molecular mechanisms involved in phototropism remain unknown, possibly because detailed physiological analyses have not been conducted properly in the isolated mutants. This chapter describes an approach for the detailed investigation of hypocotyl and root phototropism in Arabidopsis seedlings. The information provided here is expected to facilitate the analysis of phototropic responses in other plant species.
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References
Iino M (2001) Phototropism in higher plants. In: Häder D, Lebert M (eds) Photomovement. ESP comprehensive series in photosciences, vol 1. Elsevier, Amsterdam, pp 659–811
Whippo CW, Hangarter RP (2006) Phototropism: bending towards enlightenment. Plant Cell 18:1110–1119
Liscum E, Askinosie SK, Leuchtman DL, Morrow J, Willenburg KT, Coats DR (2014) Phototropism: growing towards an understanding of plant movement. Plant Cell 26:38–55
Fankhauser C, Christie JM (2015) Plant phototropic growth. Curr Biol 25:384–389
Poff KL, Janoudi AK, Rosen ES, Orbović V, Konjević R, Fortin MC, Scott TK (1994) The physiology of tropisms. In: Meyerowitz EM, Somerville CR (eds) Arabidopsis. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, pp 639–664
Christie JM, Murphy AS (2013) Shoot phototropism in higher plants: New light through old concepts. Am J Bot 100:35–46
Janoudi AK, Poff KL (1991) Characterization of adaptation in phototropism of Arabidopsis thaliana. Plant Physiol 95:517–521
Liu Y, Iino M (1996) Effects of red light on the fluence-response relationship for pulse-induced phototropism of maize coleoptiles. Plant Cell Environ 19:609–614
Liu Y, Iino M (1996) Phytochrome is required for the occurrence of time-dependent phototropism in maize coleoptiles. Plant Cell Environ 19:1379–1388
Janoudi AK, Konjević R, Whitelam G, Gordon W, Poff KL (1997) Both phytochrome A and phytochrome B are required for the normal expression of phototropism in Arabidopsis thaliana. Physiol Plant 101:278–282
Whippo CW, Hangarter RP (2004) Phytochrome modulation of blue-light-induced phototropism. Plant Cell Environ 27:1223–1228
Haga K, Sakai T (2012) PIN auxin efflux carriers are necessary for pulse-induced but not continuous light-induced phototropism in Arabidopsis. Plant Physiol 160:763–776
Haga K, Hayashi K, Sakai T (2014) PINOID AGC kinases are necessary for phytochrome-mediated enhancement of hypocotyl phototropism in Arabidopsis. Plant Physiol 166:1535–1545
Haga K, Tsuchida-Mayama T, Yamada M, Sakai T (2015) Arabidopsis ROOT PHOTOTROPISM2 contributes to the adaptation to high-intensity light in phototropic responses. Plant Cell 27:1098–1112
Sakai T, Haga K (2012) Molecular genetic analysis of phototropism in Arabidopsis. Plant Cell Physiol 53:1517–1534
Goyal A, Szarzynska B, Fankhauser C (2013) Phototropism: at the crossroads of light-signaling pathways. Trends Plant Sci 18:393–401
Christie JM, Yang H, Richter GL, Sullivan S, Thomson CE, Lin J, Titapiwatanakun B, Ennis M, Kaiserli E, Lee OR, Adamec J, Peer WA, Murphy AS (2011) phot1 inhibition of ABCB19 primes lateral auxin fluxes in the shoot apex required for phototropism. PLoS Biol 9:e1001076
Haga K, Sakai T (2013) Differential roles of auxin efflux carrier PIN proteins in hypocotyl phototropism of etiolated Arabidopsis seedlings depend on the direction of light stimulus. Plant Signal Behav 8:e22556
Kimura T, Haga K, Shimizu-Mitao Y, Takebayashi Y, Kasahara H, Hayashi K, Kakimoto T, Sakai T (2018) Asymmetric auxin distribution is not required to establish root phototropism in Arabidopsis. Plant Cell Physiol 59:828–840
Nagashima A, Uehara Y, Sakai T (2008) The ABC subfamily B auxin transporter AtABCB19 is involved in the inhibitory effects of N-1-naphthyphthalamic acid on the phototropic and gravitropic responses of Arabidopsis hypocotyls. Plant Cell Physiol 49:1250–1255
Haga K, Sakai T (2015) PINOID function in root phototropism as a negative regulator. Plant Signal Behav 10:e998545
Okada K, Shimura Y (1992) Mutational analysis of root gravitropism and phototropism of Arabidopsis thaliana seedlings. Aust J Plant Physiol 19:439–448
Iino M, Carr DJ (1981) Safelight for photomorphogenetic studies: infrared radiation and infrared-scope. Plant Sci Lett 23:263–268
Kim K, Shin J, Lee SH, Kweon HS, Maloof JN, Choi G (2011) Phytochromes inhibit hypocotyl negative gravitropism by regulating the development of endodermal amyloplasts through phytochrome-interacting factors. Proc Natl Acad Sci U S A 108:1729–1734
Fitzelle KJ, Kiss JZ (2001) Restoration of gravitropic sensitivity in starch-deficient mutants of Arabidopsis by hypergravity. J Exp Bot 52:265–275
Liscum E, Briggs WR (1995) Mutations in the NPH1 locus of Arabidopsis disrupt the perception of phototropic stimuli. Plant Cell 7:47–485
Zhang KX, Xu HH, Yuan TT, Zhang L, Lu YT (2013) Blue-light-induced PIN3 polarization for root negative phototropic response in Arabidopsis. Plant J 76:308–321
Sakai T, Wada T, Ishiguro S, Okada K (2000) RPT2: A signal transducer of the phototropic response in Arabidopsis. Plant Cell 12:225–236
Acknowledgments
We thank Professor Moritoshi Iino (Osaka City University) and the members of his laboratory for providing useful technical advice, and Professor Tatsuya Sakai (Niigata University) for providing useful comments and technical support. This work was supported by the Japan Society for the Promotion of Science (JSPS) KAKENHI (No. 24657027 and No. 17K07451 to K. H., No. 16J01942 to T. K.) and (in part) by a Grant for Basic Science Research Projects from the Sumitomo Foundation to K. H.
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Haga, K., Kimura, T. (2019). Physiological Characterization of Phototropism in Arabidopsis Seedlings. In: Yamamoto, K. (eds) Phototropism. Methods in Molecular Biology, vol 1924. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9015-3_1
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DOI: https://doi.org/10.1007/978-1-4939-9015-3_1
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