Abstract
Tissue interactions are crucial during the development of organs. Among the most studied tissue interactions are those that take place between the epithelial cells and the underlying mesenchymal cells, known as epithelial-mesenchymal interactions. Tissue recombination assay is a valuable model to study the mechanisms involved in the regulation of such interactions. Here, we describe how to dissociate and recombine the epithelial and mesenchymal components of the embryonic tooth. In addition, we explain how to transplant the recombined tissues under the kidney capsule of immunocompromised mice in order to allow their further development into a mature tooth.
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References
Jimenez-Rojo L, Granchi Z, Graf D, Mitsiadis TA (2012) Stem cell fate determination during development and regeneration of ectodermal organs. Front Physiol 3:107. https://doi.org/10.3389/fphys.2012.00107
Kollar EJ, Baird GR (1969) The influence of the dental papilla on the development of tooth shape in embryonic mouse tooth germs. J Embryol Exp Morphol 21(1):131–148
Kratochwil K (1969) Organ specificity in mesenchymal induction demonstrated in the embryonic development of the mammary gland of the mouse. Dev Biol 20(1):46–71
Mitsiadis TA, Cheraud Y, Sharpe P, Fontaine-Perus J (2003) Development of teeth in chick embryos after mouse neural crest transplantations. Proc Natl Acad Sci U S A 100(11):6541–6545. https://doi.org/10.1073/pnas.1137104100
Sakakura T, Nishizuka Y, Dawe CJ (1976) Mesenchyme-dependent morphogenesis and epithelium-specific cytodifferentiation in mouse mammary gland. Science 194(4272):1439–1441
Dhouailly D, Rogers GE, Sengel P (1978) The specification of feather and scale protein synthesis in epidermal-dermal recombinations. Dev Biol 65(1):58–68
Mitsiadis TA, Graf D, Luder H, Gridley T, Bluteau G (2010) BMPs and FGFs target Notch signalling via jagged 2 to regulate tooth morphogenesis and cytodifferentiation. Development 137(18):3025–3035. https://doi.org/10.1242/dev.049528
Kusakabe M, Sakakura T, Sano M, Nishizuka Y (1985) A pituitary-salivary mixed gland induced by tissue recombination of embryonic pituitary epithelium and embryonic submandibular gland mesenchyme in mice. Dev Biol 110(2):382–391
Caton J, Luder HU, Zoupa M, Bradman M, Bluteau G, Tucker AS, Klein O, Mitsiadis TA (2009) Enamel-free teeth: Tbx1 deletion affects amelogenesis in rodent incisors. Dev Biol 328(2):493–505. https://doi.org/10.1016/j.ydbio.2009.02.014
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Jimenez-Rojo, L., Mitsiadis, T.A. (2019). Tissue Recombination and Kidney Capsule Transplantation Assays for the Study of Epithelial-Mesenchymal Interactions. In: Papagerakis, P. (eds) Odontogenesis. Methods in Molecular Biology, vol 1922. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9012-2_6
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DOI: https://doi.org/10.1007/978-1-4939-9012-2_6
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-9011-5
Online ISBN: 978-1-4939-9012-2
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