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Scanning Electron Microscopy (SEM) Methods for Dental Enamel

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Book cover Odontogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1922))

Abstract

Scanning electron microscopy (SEM) is exceptionally well suited for the study of the structure of dental enamel, due to its ability to create high-resolution images of hard surfaces. Continuous attention on how to arrive at the observation stage with a clean and dry specimen is one main aspect of specimen preparation. Other main aspects are choice of whether the specimen should be embedded or not, choice of plane of section, and choice of acid-etching regime. Special attention is given to the preparation of small specimens and how to prepare and observe more than one plane or aspect in the same specimen.

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Authors and Affiliations

  1. Faculty of Dentistry, Institute of Oral Biology, University of Oslo, Oslo, Norway

    Steinar Risnes, Muhammad Saeed & Amer Sehic

Authors
  1. Steinar Risnes
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  2. Muhammad Saeed
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  3. Amer Sehic
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Corresponding author

Correspondence to Steinar Risnes .

Editor information

Editors and Affiliations

  1. School of Dentistry, University of Michigan, Ann Arbor, MI, USA

    Petros Papagerakis

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Risnes, S., Saeed, M., Sehic, A. (2019). Scanning Electron Microscopy (SEM) Methods for Dental Enamel. In: Papagerakis, P. (eds) Odontogenesis. Methods in Molecular Biology, vol 1922. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9012-2_27

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  • DOI: https://doi.org/10.1007/978-1-4939-9012-2_27

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9011-5

  • Online ISBN: 978-1-4939-9012-2

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