Abstract
Bioengineered dental tissues and whole teeth that exhibit features and properties of natural teeth can functionally surpass currently used artificial dental implants. However, no biologically based alternatives currently exist for clinical applications in dentistry. Here, we describe a newly established bioengineered tooth bud model for eventual applications in clinical dentistry. We also describe methods to fabricate and analyze bioengineered tooth tissues, including cell isolation, in vivo implantation, and post-harvest analyses.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Greenstein G, Cavallaro J, Romanos G, Tarnow D (2008) Clinical recommendations for avoiding and managing surgical complications associated with implant dentistry: a review. J Periodontol 79:1317–1329
Jung RE, Pjetursson BE, Glauser R, Zembic A, Zwahlen M, Lang NP (2008) A systematic review of the 5-year survival and complication rates of implant-supported single crowns. Clin Oral Implants Res 19:119–130
Chrcanovic BR, Albrektsson T, Wennerberg A (2014) Reasons for failures of oral implants. J Oral Rehabil 41:443–476
Chrcanovic BR, Kisch J, Albrektsson T, Wennerberg A (2016) Factors influencing early dental implant failures. J Dent Res 95:995–1002
Yen AH, Yelick PC (2011) Dental tissue regeneration – a mini-review. Gerontology 57:85–94
Smith EE, Yelick PC (2016) Progress in bioengineered whole tooth research: from bench to dental patient chair. Curr Oral Health Rep 3(4):302–308
Lai W-F, Lee J-M, Jung H-S (2014) Molecular and engineering approaches to regenerate and repair teeth in mammals. Cell Mol Life Sci 71:1691–1701
Monteiro N, Smith EE, Angstadt S, Zhang W, Khademhosseini A, Yelick PC (2016) Dental cell sheet biomimetic tooth bud model. Biomaterials 106:167–179
Smith EE, Zhang W, Schiele NR, Khademhosseini A, Kuo CK, Yelick PC (2017) Developing a biomimetic tooth bud model. J Tissue Eng Regen Med 11(12):3326–3336
Monteiro N, Yelick PC (2016) Advances and perspectives in tooth tissue engineering. J Tissue Eng Regen Med 11(9):2443–2461
Yue K, Santiago GT-d, Alvarez MM, Tamayol A, Annabi N, Khademhosseini A (2015) Synthesis, properties, and biomedical applications of gelatin methacryloyl (GelMA) hydrogels. Biomaterials 73:254–271
Nichol JW, Koshy ST, Bae H, Hwang CM, Yamanlar S, Khademhosseini A (2010) Cell-laden microengineered gelatin methacrylate hydrogels. Biomaterials 31:5536–5544
Acknowledgments
All members of the Yelick Tissue Engineering Lab have contributed to optimize and validate these techniques. This work was supported by NIH/NIDCR R01 DE16132 (PCY) and NIH/NIDCR F31 DE026361 (EES).
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2019 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Smith, E.E., Yelick, P.C. (2019). Bioengineering Tooth Bud Constructs Using GelMA Hydrogel. In: Papagerakis, P. (eds) Odontogenesis. Methods in Molecular Biology, vol 1922. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9012-2_14
Download citation
DOI: https://doi.org/10.1007/978-1-4939-9012-2_14
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-9011-5
Online ISBN: 978-1-4939-9012-2
eBook Packages: Springer Protocols