Abstract
Bone histomorphometry remains an important tool with which to study the pathophysiology of bone disease and the cellular mechanism by which treatments work. Here we review the methods for embedding, sectioning, staining, and analysis of bone sections in rodents.
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References
Dempster DW, Compston JE, Drezner MK, Glorieux FH, Kanis JA, Malluche H et al (2013) Standardized nomenclature, symbols, and units for bone histomorphometry: a 2012 update of the report of the ASBMR Histomorphometry Nomenclature Committee. J Bone Miner Res 28:2–17
Erben RG (1997) Embedding of bone samples in methylmethacrylate: an improved method suitable for bone histomorphometry, histochemistry, and immunohistochemistry. J Histochem Cytochem 45:307–313
Schenk RK, Olah AJ, Herrmann W (1984) Preparation of calcified tissues for light microscopy. In: Dickson GR (ed) Methods of calcified tissue preparation. Elsevier, Amsterdam, pp 1–56
Reim NS, Breig B, Stahr K, Eberle J, Hoeflich A, Wolf E et al (2008) Cortical bone loss in androgen-deficient aged male rats is mainly caused by increased endocortical bone remodeling. J Bone Miner Res 23:694–704
Baron R, Vignery A, Neff L, Silverglate A, Santa Maria A (1983) Processing of undecalcified bone specimens for bone histomorphometry. In: Recker RR (ed) Bone histomorphometry: techniques and interpretation. CRC Press, Boca Raton, FL, pp 13–35
Erben RG (2003) Bone labeling techniques. In: An YH, Martin KL (eds) Handbook of histology methods for bone and cartilage. Humana Press Inc., Totowa, NJ, pp 99–117
Erben RG, Scutt AM, Miao DS, Kollenkirchen U, Haberey M (1997) Short-term treatment of rats with high dose 1,25-dihydroxyvitamin D3 stimulates bone formation and increases the number of osteoblast precursor cells in bone marrow. Endocrinology 138:4629–4635
Frost HM (1983) Bone histomorphometry: choice of marking agent and labeling schedule. In: Recker RR (ed) Bone histomorphometry: techniques and interpretation. CRC Press, Boca Raton, Florida, pp 37–52
Frost HM (1983) Bone histomorphometry: analysis of trabecular bone dynamics. In: Recker RR (ed) Bone histomorphometry: techniques and interpretation. CRC Press, Boca Raton, Florida, pp 109–131
Erben RG (1996) Trabecular and endocortical bone surfaces in the rat: modeling or remodeling? Anat Rec 246:39–46
Bentolila V, Boyce TM, Fyhrie DP, Drumb R, Skerry TM, Schaffler MB (1998) Intracortical remodeling in adult rat long bones after fatigue loading. Bone 23:275–281
Ibbotson KJ, Orcutt CM, D'Souza SM, Paddock CL, Arthur JA, Jankowsky ML et al (1992) Contrasting effects of parathyroid hormone and insulin-like growth factor I in an aged ovariectomized rat model of postmenopausal osteoporosis. J Bone Miner Res 7:425–432
Lauritzen DB, Balena R, Shea M, Seedor JG, Markatos A, Le HM et al (1993) Effects of combined prostaglandin and alendronate treatment on the histomorphometry and biomechanical properties of bone in ovariectomized rats. J Bone Miner Res 8:871–879
Uzawa T, Hori M, Ejiri S, Ozawa H (1995) Comparison of the effects of intermittent and continuous administration of human parathyroid hormone(1-34) on rat bone. Bone 16:477–484
Weber K, Kaschig C, Erben RG (2004) 1alpha-Hydroxyvitamin D2 and 1alpha-hydroxyvitamin D3 have anabolic effects on cortical bone, but induce intracortical remodeling at toxic doses in ovariectomized rats. Bone 35:704–710
Erben RG, Eberle J, Stahr K, Goldberg M (2000) Androgen deficiency induces high turnover osteopenia in aged male rats: a sequential histomorphometric study. J Bone Miner Res 15:1085–1098
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Erben, R.G., Glösmann, M. (2019). Histomorphometry in Rodents. In: Idris, A. (eds) Bone Research Protocols. Methods in Molecular Biology, vol 1914. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8997-3_24
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DOI: https://doi.org/10.1007/978-1-4939-8997-3_24
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