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In Vivo Models of Mechanical Loading

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Bone Research Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1914))

Abstract

The skeleton fulfils its mechanical functions through structural organization and material properties of individual bones. It is stated that both cortical and trabecular morphology and mass can be (re)modelled in response to changes in mechanical strains engendered by load-bearing. To address this, animal models that enable the application of specific loads to individual bones have been developed. These are useful in defining how loading modulates (re)modeling and allow examination of the mechanisms that coordinate these events. This chapter describes how to apply mechanical loading to murine bones through points of articulation, which allows changes in endosteal, periosteal as well as trabecular bone to be revealed at multiple hierarchies, by a host of methodologies, including double fluorochrome labeling and computed tomography.

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Author information

Authors and Affiliations

  1. Skeletal Biology Group, Comparative Biomedical Sciences, The Royal Veterinary College, London, UK

    Behzad Javaheri & Andrew A. Pitsillides

  2. Department Clinical Chemistry, VU University Medical Center, Amsterdam, The Netherlands

    Nathalie Bravenboer

  3. Center for Bone Quality and Department of Internal Medicine, Division Endocrinology, Leiden University Medical Center, Leiden, The Netherlands

    Nathalie Bravenboer

  4. Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam, Amsterdam, The Netherlands

    Astrid D. Bakker

  5. Department of Physics and Medical Technology, VU University Medical Center, Amsterdam, The Netherlands

    Albert van der Veen

  6. Departamento de Clínica, Universidade Federal de Mato Grosso (UFMT), Cuiabá, Brazil

    Roberto Lopes de Souza

  7. University College London, London, UK

    Leanne Saxon

Authors
  1. Behzad Javaheri
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  2. Nathalie Bravenboer
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  3. Astrid D. Bakker
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  4. Albert van der Veen
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  5. Roberto Lopes de Souza
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  6. Leanne Saxon
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  7. Andrew A. Pitsillides
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Corresponding author

Correspondence to Andrew A. Pitsillides .

Editor information

Editors and Affiliations

  1. Department of Oncology and Metabolism, University of Sheffield, Sheffield, UK

    Aymen I. Idris

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Javaheri, B. et al. (2019). In Vivo Models of Mechanical Loading. In: Idris, A. (eds) Bone Research Protocols. Methods in Molecular Biology, vol 1914. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8997-3_22

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  • DOI: https://doi.org/10.1007/978-1-4939-8997-3_22

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8996-6

  • Online ISBN: 978-1-4939-8997-3

  • eBook Packages: Springer Protocols

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