Skip to main content
SpringerLink
Log in
Book cover

Bone Research Protocols pp 261–267Cite as

Ovariectomy/Orchiectomy in Rodents

  • Protocol
  • First Online:

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1914))

Abstract

This chapter describes the surgical procedures for ovariectomy and orchiectomy in mice and rats. In addition to providing technical details of the surgical techniques, details of anesthesia options and pre-, peri-, and postoperative care are also included.

This is a preview of subscription content, log in via an institution.

Protocol
USD   49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   189.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book
USD   249.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

References

  1. Khosla S, Melton LJ III, Riggs BL (2001) Estrogens and bone health in men. Calcif Tissue Int 69:189–192

    Article  CAS  Google Scholar 

  2. Khosla S, Melton LJ III, Atkinson EJ, O’Fallon WM, Klee GG, Riggs BL (1998) Relationship of serum sex steroid levels and bone turnover markers with bone mineral density in men and women: a key role for bioavailable estrogen. J Clin Endocrinol Metab 83:2266–2274

    CAS  PubMed  Google Scholar 

  3. Samuels A, Perry MJ, Tobias JH (1999) High-dose estrogen induces de novo medullary bone formation in female mice. J Bone Miner Res 14:178–186

    Article  CAS  Google Scholar 

  4. Tobias JH, Compston JE (1999) Does estrogen stimulate osteoblast function in postmenopausal women? Bone 24:121–124

    Article  CAS  Google Scholar 

  5. Weinstein RS, Manolagas SC (2000) Apoptosis and osteoporosis. Am J Med 108:153–164

    Article  CAS  Google Scholar 

  6. Raisz LG (2005) Pathogenesis of osteoporosis: concepts, confl icts, and prospects. J Clin Invest 115:3318–3325

    Article  CAS  Google Scholar 

  7. Idris AI, Van’t Hof RJ, Greig IR, Ridge SA, Baker D, Ross RA, Ralston SH (2005) Regulation of bone mass, bone loss and osteoclast activity by cannabinoid receptors. Nat Med 11:774–779

    Article  CAS  Google Scholar 

  8. Rissanen JP, Suominen MI, Peng Z, Morko J, Rasi S, Risteli J, Halleen JM (2008) Short-term changes in serum PINP predict long-term changes in trabecular bone in the rat ovariectomy model. Calcif Tissue Int 82:155–161

    Article  CAS  Google Scholar 

  9. Sinnesael M, Boonen S, Claessens F, Gielen E, Vanderschueren D (2011) Testosterone and the male skeleton: a dual mode of action. J Osteoporos 2011:240328

    Article  Google Scholar 

  10. Gunness M, Orwoll E (1995) Early induction of alterations in cancellous and cortical bone histology after orchiectomy in mature rats. J Bone Miner Res 10:1735–1744

    Article  CAS  Google Scholar 

  11. Danielsen CC, Mosekilde L, Andreassen TT (1992) Long-term effect of orchidectomy on cortical bone from rat femur: bone mass and mechanical properties. Calcif Tissue Int 50:169–174

    Article  CAS  Google Scholar 

  12. Orwoll ES (1996) Androgens as anabolic agents for bone. Trends Endocrinol Metab 7:77–84

    Article  CAS  Google Scholar 

  13. Gaertner DJ, Hallman TM, Hankenson FC, Batchelder MA (2008) Anesthesia and analgesia for laboratory rodents. In: Fish RE, Brown MJ, Danneman PJ, Karas AZ (eds) Anesthesia and analgesia in laboratory animals, American College of Laboratory Animal medicine series. Academic Press, San Diego, pp 239–297

    Chapter  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Life Sciences, School of Sciences, European University Cyprus, Nicosia, Cyprus

    Antonia Sophocleous

  2. Department of Oncology and Metabolism, University of Sheffield, Sheffield, UK

    Aymen I. Idris

Authors
  1. Antonia Sophocleous
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Aymen I. Idris
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Aymen I. Idris .

Editor information

Editors and Affiliations

  1. Department of Oncology and Metabolism, University of Sheffield, Sheffield, UK

    Aymen I. Idris

Rights and permissions

Reprints and permissions

Copyright information

© 2019 Springer Science+Business Media, LLC, part of Springer Nature

About this protocol

Check for updates. Verify currency and authenticity via CrossMark

Cite this protocol

Sophocleous, A., Idris, A.I. (2019). Ovariectomy/Orchiectomy in Rodents. In: Idris, A. (eds) Bone Research Protocols. Methods in Molecular Biology, vol 1914. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8997-3_13

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-8997-3_13

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8996-6

  • Online ISBN: 978-1-4939-8997-3

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation